ArticleThe journal of physical chemistry letters2014
Single-Molecule Fluorescence Using Nucleotide Analogs: A Proof-of-Principle.
Article in The journal of physical chemistry letters, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 34 citations in OpenAlex.
- Force-induced melting and S-DNA pathways for DNA overstretching exhibit distinct kinetics.Nucleic acids research · 2025Article
- Mechanical characterization of base analogue modified nucleic acids by force spectroscopy.Physical chemistry chemical physics : PCCP · 2021Article
- Single-molecule fluorescence detection of a tricyclic nucleoside analogue.Chemical science · 2020Article
- Single-Molecule Detection of a Fluorescent Nucleobase Analogue via Multiphoton Excitation.The journal of physical chemistry letters · 2019Article
- Recent progress in dissecting molecular recognition by DNA polymerases with non-native substrates.Current opinion in chemical biology · 2017Review
- Synthesis and Optoelectronic Properties of Thiophene Donor and Thiazole Acceptor Based Blue Fluorescent Conjugated Oligomers.Journal of fluorescence · 2016Article
- Studying Z-DNA and B- to Z-DNA transitions using a cytosine analogue FRET-pair.Nucleic acids research · 2016Article
- Studies of Luminescence Performance on Carbazole Donor and Quinoline Acceptor Based Conjugated Polymer.Journal of fluorescence · 2016Article
- Development of bright fluorescent quadracyclic adenine analogues: TDDFT-calculation supported rational design.Scientific reports · 2015Article
- Single-molecule tools for enzymology, structural biology, systems biology and nanotechnology: an update.Archives of toxicology · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Fluorescent nucleotide analogues, such as 2-aminopurine (2AP) and pyrrolo-C (PyC), have been extensively used to study nucleic acid local conformational dynamics in bulk experiments. Here we present a proof-of-principle approach using 2AP and PyC fluorescence at the single-molecule level. Our data show that ssDNA, dsDNA, or RNA containing both 2AP and PyC can be monitored using single-molecule fluorescence and a click chemistry immobilization method. We demonstrate that this approach can be used to monitor DNA and RNA in real time. This is the first reported assay using fluorescent nucleotide analogs at the single-molecule level. We anticipate that single 2AP or PyC fluorescence will have numerous applications in studies of DNA and RNA, including protein-induced base-flipping dynamics in protein-nucleic acid complexes.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.