ArticleApplied physics letters2014
Label-free, real-time detection of the dynamic processes of protein degradation using oblique-incidence reflectivity difference method.
Article in Applied physics letters, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 15 citations in OpenAlex.
- Moiré Lattice of Twisted Bilayer Graphene as Template for Non-Covalent Functionalization.Angewandte Chemie (International ed. in English) · 2025Article
- Gold nanoparticle-decorated fluorine-doped tin oxide substrate for sensitive label-free OIRD microarray chips.Analytical and bioanalytical chemistry · 2024Article
- Real-time detection of dielectric anisotropy or isotropy in unconventional oil-gas reservoir rocks supported by the oblique-incidence reflectivity difference technique.Scientific reports · 2016Article
- Developing an Efficient and General Strategy for Immobilization of Small Molecules onto Microarrays Using Isocyanate Chemistry.Sensors (Basel, Switzerland) · 2016Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
Based on the requirements for studying the dynamic process of proteinase action substrates in life science, we selected six random proteins including 1L-10, SCGB2A2, CENPQ, GST, HK1, KLHL7, as well as five different concentrations of 1L-10 proteins of 1 mg/ml, 0.5 mg/ml, 0.25 mg/ml, 0.125 mg/ml, and 0.0625 mg/ml, and fabricated two types of substrate protein microarrays, respectively. We detected the dynamic processes of proteins degraded by proteinase K using oblique-incidence reflectivity difference (OIRD) method in a label-free and real-time manner. We obtained the relevant degradation velocities and the degradation time. The experimental results demonstrate that OIRD has the ability to study proteinase action substrates which is out of reach of label methods and is expected to offer opportunities to determine protease-substrate relationships on the systems biology level.
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Registered trials
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