ArticleNucleic acids research2014
Development of synthetic selfish elements based on modular nucleases in Drosophila melanogaster.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 37 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 75 citations in OpenAlex.
- Genetic engineering for SIT application: a fruit fly-focused review.Insect science · 2026Review
- Advances in CRISPR gene drives for mosquito population control.Current opinion in microbiology · 2026Review
- Germline Cas9 promoters with improved performance for homing gene drive.Nature communications · 2024Article
- A small-molecule approach to restore female sterility phenotype targeted by a homing suppression gene drive in the fruit pest Drosophila suzukii.PLoS genetics · 2024Article
- Manipulating the Destiny of Wild Populations Using CRISPR.Annual review of genetics · 2023Review
- Gene drive designs for efficient and localisable population suppression using Y-linked editors.PLoS genetics · 2022Article
- A CRISPR endonuclease gene drive reveals distinct mechanisms of inheritance bias.Nature communications · 2022Article
- Reflection on the Challenges, Accomplishments, and New Frontiers of Gene Drives.Biodesign research · 2022Review
- Driving down malaria transmission with engineered gene drives.Frontiers in genetics · 2022Review
- Intronic gRNAs for the Construction of Minimal Gene Drive Systems.Frontiers in bioengineering and biotechnology · 2022Article
- The Challenges in Developing Efficient and Robust Synthetic Homing Endonuclease Gene Drives.Frontiers in bioengineering and biotechnology · 2022Review
- A New Approach to Develop Resistant Cultivars Against the Plant Pathogens: CRISPR Drives.Frontiers in plant science · 2022Article
- Modeling-informed Engineered Genetic Incompatibility strategies to overcome resistance in the invasiveFrontiers in insect science · 2022Article
- A genetically encoded anti-CRISPR protein constrains gene drive spread and prevents population suppression.Nature communications · 2021Article
- Double drives and private alleles for localised population genetic control.PLoS genetics · 2021Article
- Adequacy and sufficiency evaluation of existing EFSA guidelines for the molecular characterisation, environmental risk assessment and post-market environmental monitoring of genetically modified insects containing engineered gene drives.EFSA journal. European Food Safety Authority · 2020Article
- Resistance to natural and synthetic gene drive systems.Journal of evolutionary biology · 2020Review
- Gene drives in plants: opportunities and challenges for weed control and engineered resilience.Proceedings. Biological sciences · 2019Article
- Daisy-chain gene drives for the alteration of local populations.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Cleave and Rescue, a novel selfish genetic element and general strategy for gene drive.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Selfish genes are DNA elements that increase their rate of genetic transmission at the expense of other genes in the genome and can therefore quickly spread within a population. It has been suggested that selfish elements could be exploited to modify the genome of entire populations for medical and ecological applications. Here we report that transcription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) can be engineered into site-specific synthetic selfish elements (SSEs) and demonstrate their transmission of up to 70% in the Drosophila germline. We show here that SSEs can spread via DNA break-induced homologous recombination, a process known as 'homing' similar to that observed for homing endonuclease genes (HEGs), despite their fundamentally different modes of DNA binding and cleavage. We observed that TALEN and ZFN have a reduced capability of secondary homing compared to HEG as their repetitive structure had a negative effect on their genetic stability. The modular architecture of ZFNs and TALENs allows for the rapid design of novel SSEs against specific genomic sequences making them potentially suitable for the genetic engineering of wild-type populations of animals and plants, in applications such as gene replacement or population suppression of pest species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.