ArticleMolecular biology and evolution2014
Bulk de novo mitogenome assembly from pooled total DNA elucidates the phylogeny of weevils (Coleoptera: Curculionoidea).
Article in Molecular biology and evolution, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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85 citing papers in PubMed.
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- Phylogenetic relationships and divergence times of Odonata inferred from mitochondrial genome.iScience · 2025Article
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- Navigating uncertainty in museum workflows: genomic data mining and curation of the Diptera collections hosted at RMCA.Biodiversity data journal · 2025Article
- Comparative Analysis of the Mitochondrial Genomes of Chloropidae and Their Implications for the Phylogeny of the Family.International journal of molecular sciences · 2024Article
- Genetic characterization of 2 Ceutorhynchus (Coleoptera: Curculionidae) weevils with mitogenomes and insights into the phylogeny and evolution of related weevils.Journal of insect science (Online) · 2024Article
- Mitochondrial genomes provide insights into the Euholognatha (Insecta: Plecoptera).BMC ecology and evolution · 2024Article
- The complete mitochondrial genome ofMitochondrial DNA. Part B, Resources · 2024Article
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- In silico prospecting of the mtDNA of Macrobrachium amazonicum from transcriptome data.BMC genomics · 2023Article
- Novel tRNA Gene Rearrangements in the Mitochondrial Genomes of Poneroid Ants and Phylogenetic Implication of Paraponerinae (Hymenoptera: Formicidae).Life (Basel, Switzerland) · 2023Article
25 more citing papers are in PubMed but not listed here.
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6 authors.
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Abstract
Complete mitochondrial genomes have been shown to be reliable markers for phylogeny reconstruction among diverse animal groups. However, the relative difficulty and high cost associated with obtaining de novo full mitogenomes have frequently led to conspicuously low taxon sampling in ensuing studies. Here, we report the successful use of an economical and accessible method for assembling complete or near-complete mitogenomes through shot-gun next-generation sequencing of a single library made from pooled total DNA extracts of numerous target species. To avoid the use of separate indexed libraries for each specimen, and an associated increase in cost, we incorporate standard polymerase chain reaction-based "bait" sequences to identify the assembled mitogenomes. The method was applied to study the higher level phylogenetic relationships in the weevils (Coleoptera: Curculionoidea), producing 92 newly assembled mitogenomes obtained in a single Illumina MiSeq run. The analysis supported a separate origin of wood-boring behavior by the subfamilies Scolytinae, Platypodinae, and Cossoninae. This finding contradicts morphological hypotheses proposing a close relationship between the first two of these but is congruent with previous molecular studies, reinforcing the utility of mitogenomes in phylogeny reconstruction. Our methodology provides a technically simple procedure for generating densely sampled trees from whole mitogenomes and is widely applicable to groups of animals for which bait sequences are the only required prior genome knowledge.
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