ArticleThe Journal of cell biology1989
Monoclonal antibodies detect and stabilize conformational states of smooth muscle myosin.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 45 citations in OpenAlex.
- Evolution and biological characteristics of H11 avian influenza viruses isolated from migratory birds and pigeons.Emerging microbes & infections · 2024Article
- Filament evanescence of myosin II and smooth muscle function.The Journal of general physiology · 2021Review
- Myosin filaments in smooth muscle cells do not have a constant length.The Journal of physiology · 2013Article
- A mutant heterodimeric myosin with one inactive head generates maximal displacement.The Journal of cell biology · 2003Article
- Conformational changes in the herpes simplex virus ICP8 DNA-binding protein coincident with assembly in viral replication structures.Journal of virology · 2003Article
- The biochemical kinetics underlying actin movement generated by one and many skeletal muscle myosin molecules.Biophysical journal · 2002Article
- Myosin V exhibits a high duty cycle and large unitary displacement.The Journal of cell biology · 2001Article
- Slow cycling of unphosphorylated myosin is inhibited by calponin, thus keeping smooth muscle relaxed.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Antibodies probe for folded monomeric myosin in relaxed and contracted smooth muscle.The Journal of cell biology · 1994Article
- Coupling of ATPase activity and motility in smooth muscle myosin is mediated by the regulatory light chain.The Journal of cell biology · 1994Article
- Distribution of developmental myosin isoforms in isolated A-segments.Journal of muscle research and cell motility · 1992Article
- Probing myosin light chain 1 structure with monoclonal antibodies.Journal of muscle research and cell motility · 1992Article
- Identification of functional regions on the tail of Acanthamoeba myosin-II using recombinant fusion proteins. I. High resolution epitope mapping and characterization of monoclonal antibody binding sites.The Journal of cell biology · 1990Article
- Smooth muscle myosin cross-bridge interactions modulate actin filament sliding velocity in vitro.The Journal of cell biology · 1990Article
- Filamentous smooth muscle myosin is regulated by phosphorylation.The Journal of cell biology · 1989Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Antibodies with epitopes near the heavy meromyosin/light meromyosin junction distinguish the folded from the extended conformational states of smooth muscle myosin. Antibody 10S.1 has 100-fold higher avidity for folded than for extended myosin, while antibody S2.2 binds preferentially to the extended state. The properties of these antibodies provide direct evidence that the conformation of the rod is different in the folded than the extended monomeric state, and suggest that this perturbation may extend into the subfragment 2 region of the rod. Two antihead antibodies with epitopes on the heavy chain map at or near the head/rod junction. Magnesium greatly enhances the binding of these antibodies to myosin, showing that the conformation of the heavy chain in the neck region changes upon divalent cation binding to the regulatory light chain. Myosin assembly is also altered by antibody binding. Antibodies that bind to the central region of the rod block disassembly of filaments upon MgATP addition. Antibodies with epitopes near the COOH terminus of the rod, in contrast, promote filament depolymerization, suggesting that this region of the tail is important for assembly. The monoclonal antibodies described here are therefore useful both for detecting and altering conformational states of smooth muscle myosin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.