ArticleJournal of experimental botany2014
Fusion, rupture, and degeneration: the fate of in vivo-labelled PSVs in developing barley endosperm.
Article in Journal of experimental botany, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Mechanisms of Differential Resource Uptake and Translocation in Agaricus bisporus.Environmental microbiology · 2026Article
- Functional Analysis of the Autophagy-Related GenePlants (Basel, Switzerland) · 2025Article
- Automated mass spectrometry-based profiling of multi-glycosylated glycosyl inositol phospho ceramides (GIPC) reveals specific series GIPC rearrangements during barley grain development and heat stress response.The Plant journal : for cell and molecular biology · 2025Article
- Immunolocalization of hordein synthesis and transport in developing barley endosperm.Plant direct · 2024Article
- Imaging the ER and Endomembrane System in Cereal Endosperm.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Multiscale imaging reveals the presence of autophagic vacuoles in developing maize endosperm.Frontiers in plant science · 2022Article
- Tissue-Specific Proteome and Subcellular Microscopic Analyses Reveal the Effect of High Salt Concentration on Actin Cytoskeleton and Vacuolization in Aleurone Cells during Early Germination of Barley.International journal of molecular sciences · 2021Article
- Protein sorting into protein bodies during barley endosperm development is putatively regulated by cytoskeleton members, MVBs and the HvSNF7s.Scientific reports · 2020Article
- Multiple strategies for heat adaptation to prevent chalkiness in the rice endosperm.Journal of experimental botany · 2019Article
- Hordein Accumulation in Developing Barley Grains.Frontiers in plant science · 2019Article
- Microscopic and Proteomic Analysis of Dissected Developing Barley Endosperm Layers Reveals the Starchy Endosperm as Prominent Storage Tissue for ER-Derived Hordeins Alongside the Accumulation of Barley Protein Disulfide Isomerase (HvPDIL1-1).Frontiers in plant science · 2018Article
- Using RT-qPCR, Proteomics, and Microscopy to Unravel the Spatio-Temporal Expression and Subcellular Localization of Hordoindolines Across Development in Barley Endosperm.Frontiers in plant science · 2018Article
- Cell layer-specific distribution of transiently expressed barley ESCRT-III component HvVPS60 in developing barley endosperm.Protoplasma · 2016Article
- Abscisic acid prevents the coalescence of protein storage vacuoles by upregulating expression of a tonoplast intrinsic protein gene in barley aleurone.Journal of experimental botany · 2015Article
- Live Cell Imaging During Germination Reveals Dynamic Tubular Structures Derived from Protein Storage Vacuoles of Barley Aleurone Cells.Plants (Basel, Switzerland) · 2014Article
- The dynamic behavior of storage organelles in developing cereal seeds and its impact on the production of recombinant proteins.Frontiers in plant science · 2014Review
Corrections and comments
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Authors and funding
5 authors.
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Abstract
Cereal endosperm is a highly differentiated tissue containing specialized organelles for the accumulation of storage proteins. The endosperm of barley contains hordeins, which are ultimately deposited within protein storage vacuoles (PSVs). These organelles have been characterized predominantly by the histochemical analysis of fixed immature tissue samples. However, little is known about the fate of PSVs during barley endosperm development, and in vivo imaging has not been attempted in order to gain further insight. In this report, young seeds were followed through development to characterize the dynamic morphology of PSVs from aleurone, subaleurone, and central starchy endosperm cells. TIP3-GFP was used as a PSV membrane marker and several fluorescent tracers were used to identify membranes and monitor endomembrane organelles in real time. Whereas the spherical appearance of strongly labelled TIP3-GFP PSVs in the aleurone remained constant, those in the subaleurone and central starchy endosperm underwent substantial morphological changes. Fusion and rupture events were observed in the subaleurone, and internal membranes derived from both the tonoplast and endoplasmic reticulum were identified within these PSVs. TIP3-GFP-labelled PSVs in the starchy endosperm cells underwent a dramatic reduction in size, so that finally the protein bodies were tightly enclosed. Potential desiccation-related membrane-altering processes that may be causally linked to these dynamic endomembrane events in the barley endosperm are discussed.
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