ArticleReproduction (Cambridge, England)2014
De novo morphogenesis of testis tissue: an improved bioassay to investigate the role of VEGF165 during testis formation.
Article in Reproduction (Cambridge, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Germ cells are essential for testicular morphogenesis and functional reconstruction in a porcine xenograft model.Scientific reports · 2026Article
- Optimizing Immature Testicular Tissue and Cell Transplantation Results: Comparing Transplantation Sites and Scaffolds.International journal of fertility & sterility · 2023Review
- Male fertility preservation and restoration strategies for patients undergoing gonadotoxic therapies†.Biology of reproduction · 2022Review
- Review
- Fertility Preservation and Restoration Options for Pre-Pubertal Male Cancer Patients: Current Approaches.Frontiers in endocrinology · 2022Review
- Brief exposure of neonatal testis cells to EGF or GDNF alters the regenerated tissue.Reproduction & fertility · 2022Article
- Review
- A reduction of primary cilia but not hedgehog signaling disrupts morphogenesis in testicular organoids.Cell and tissue research · 2020Article
- Three-dimensional testicular organoids as novelEnvironmental epigenetics · 2019Review
- TALEN-mediated gene targeting in porcine spermatogonia.Molecular reproduction and development · 2018Article
- Efficient generation of functional haploid spermatids from human germline stem cells by three-dimensional-induced system.Cell death and differentiation · 2018Article
- An overview of a Sertoli cell transplantation model to study testis morphogenesis and the role of the Sertoli cells in immune privilege.Environmental epigenetics · 2017Review
- Xenografting of isolated equine (Equus caballus) testis cells results in de novo morphogenesis of seminiferous tubules but not spermatogenesis.Andrology · 2017Article
- Multiplex shRNA Screening of Germ Cell Development by in Vivo Transfection of Mouse Testis.G3 (Bethesda, Md.) · 2017Article
- Regeneration of Bovine Mammary Gland in Immunodeficient Mice by Transplantation of Bovine Mammary Epithelial Cells Mixed with Matrigel.International journal of stem cells · 2016Article
- Spermatogonial stem cells: Current biotechnological advances in reproduction and regenerative medicine.World journal of stem cells · 2015Article
- Stirred suspension bioreactors as a novel method to enrich germ cells from pre-pubertal pig testis.Andrology · 2015Article
- Beyond the mouse monopoly: studying the male germ line in domestic animal models.ILAR journal · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
De novo formation of testis tissue from single-cell suspensions allows manipulation of different testicular compartments before grafting to study testicular development and the spermatogonial stem cell niche. However, the low percentages of newly formed seminiferous tubules supporting complete spermatogenesis and lack of a defined protocol have limited the use of this bioassay. Low spermatogenic efficiency in de novo formed tissue could result from the scarcity of germ cells in the donor cell suspension, cell damage caused by handling or from hypoxia during tissue formation in the host environment. In this study, we compared different proportions of spermatogonia in the donor cell suspension and the use of Matrigel as a scaffold to support de novo tissue formation and spermatogenesis. Then, we used the system to investigate the role of vascular endothelial growth factor 165 (VEGF165) during testicular morphogenesis on blood vessel and seminiferous tubule formation, and on presence of germ cells in the de novo developed tubules. Our results show that donor cell pellets with 10×10(6) porcine neonatal testicular cells in Matrigel efficiently formed testis tissue de novo. Contrary to what was expected, the enrichment of the cell suspension with germ cells did not result in higher numbers of tubules supporting spermatogenesis. The addition of VEGF165 did not improve blood vessel or tubule formation, but it enhanced the number of tubules containing spermatogonia. These results indicate that spermatogenic efficiency was improved by the addition of Matrigel, and that VEGF165 may have a protective role supporting germ cell establishment in their niche.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.