ArticlePlant molecular biology2014
Virus induced gene silencing of three putative prolyl 4-hydroxylases enhances plant growth in tomato (Solanum lycopersicum).
Article in Plant molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 35 citations in OpenAlex.
- Splicing regulation by RS2Z36 controls ovary patterning and fruit growth in tomato.Plant molecular biology · 2026Article
- Efficient Virus-Induced Gene Silencing (VIGS) Method for Discovery of Resistance Genes in Soybean.Plants (Basel, Switzerland) · 2025Article
- Genome-wide identification of SlIQMs and the regulatory effect of calcium on tomato seedlings under drought stress and phytohormone treatment.Plant cell reports · 2025Article
- Article
- The modified activity of prolyl 4 hydroxylases reveals the effect of arabinogalactan proteins on changes in the cell wall during the tomato ripening process.Frontiers in plant science · 2024Article
- Natural variation in the prolyl 4-hydroxylase gene PtoP4H9 contributes to perennial stem growth in Populus.The Plant cell · 2023Article
- Article
- Review: structure and modifications of arabinogalactan proteins (AGPs).BMC plant biology · 2023Review
- Arabinogalactan Proteins: Focus on the Role in Cellulose Synthesis and Deposition during Plant Cell Wall Biogenesis.International journal of molecular sciences · 2022Review
- Sweet Modifications Modulate Plant Development.Biomolecules · 2021Review
- Prolyl Hydroxylase Paralogs inFrontiers in plant science · 2021Article
- The role of arabinogalactan proteins (AGPs) in fruit ripening-a review.Horticulture research · 2020Review
- Extensin arabinosylation is involved in root response to elicitors and limits oomycete colonization.Annals of botany · 2020Article
- Plant Cell Walls Tackling Climate Change: Insights into Plant Cell Wall Remodeling, Its Regulation, and Biotechnological Strategies to Improve Crop Adaptations and Photosynthesis in Response to Global Warming.Plants (Basel, Switzerland) · 2020Review
- The Contribution of Plant Dioxygenases to Hypoxia Signaling.Frontiers in plant science · 2020Review
- The Spatio-Temporal Distribution of Cell Wall-Associated Glycoproteins During Wood Formation inFrontiers in plant science · 2020Article
- On the Potential Function of Type II ArabinogalactanFrontiers in plant science · 2020Review
- Suppression of a Prolyl 4 Hydroxylase Results in Delayed Abscission of Overripe Tomato Fruits.Frontiers in plant science · 2019Article
- Pyridine 2,4-Dicarboxylic Acid Suppresses Tomato Seedling Growth.Frontiers in chemistry · 2018Article
- Virus-induced plasma membrane aquaporin PsPIP2;1 silencing inhibits plant water transport of Pisum sativum.Botanical studies · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proline hydroxylation is a major posttranslational modification of hydroxyproline-rich glycoproteins (HRGPs) that is catalyzed by prolyl 4-hydroxylases (P4Hs). HRGPs such as arabinogalactan proteins (AGPs) and extensios play significant roles on cell wall structure and function and their implication in cell division and expansion has been reported. We used tobacco rattle virus (TRV)-based virus induced gene silencing to investigate the role of three tomato P4Hs, out of ten present in the tomato genome, in growth and development. Eight-days old tomato seedlings were infected with the appropriate TRV vectors and plants were allowed to grow under standard conditions for 6 weeks. Lower P4H mRNA levels were associated with lower hydroxyproline content in root and shoot tissues indicating successful gene silencing. P4H-silenced plants had longer roots and shoots and larger leaves. The increased leaf area can be attributed to increased cell division as indicated by the higher leaf epidermal cell number in SlP4H1- and SlP4H9-silenced plants. In contrast, SlP4H7-silenced plants had larger leaves due to enhanced cell expansion. Western blot analysis revealed that silencing of SlP4H7 and SlP4H9 was associated with reduced levels of JIM8-bound AGP and JIM11-bound extensin epitopes, while silencing of SlP4H1 reduced only the levels of AGP proteins. Collectively these results show that P4Hs have significant and distinct roles in cell division and expansion of tomato leaves.
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