ArticleTissue engineering. Part C, Methods2015
Lowered humidity produces human epidermal equivalents with enhanced barrier properties.
Article in Tissue engineering. Part C, Methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Next-generation full-thickness human skin models produced using 3D electrospun scaffolds and animal-component-free culture media.Frontiers in toxicology · 2026Article
- Flexible surface acoustic wave technology for enhancing transdermal drug delivery.Drug delivery and translational research · 2025Article
- Down-regulation of peptidylarginine deiminase type 1 in reconstructed human epidermis disturbs nucleophagy in the granular layer and affects barrier function.Cell death discovery · 2023Article
- Adapting Clofazimine for Treatment of Cutaneous Tuberculosis by Using Self-Double-Emulsifying Drug Delivery Systems.Antibiotics (Basel, Switzerland) · 2022Review
- Epidermal Basement Membrane Substitutes for Bioengineering of Human Epidermal Equivalents.JID innovations : skin science from molecules to population health · 2022Article
- Keratinocyte-derived IL-1β induces PPARG downregulation and PPARD upregulation in human reconstructed epidermis following barrier impairment.Experimental dermatology · 2021Article
- Unravelling effects of relative humidity on lipid barrier formation in human skin equivalents.Archives of dermatological research · 2019Article
- Review
- Mathematical-model-guided development of full-thickness epidermal equivalent.Scientific reports · 2018Article
- Aquatide Activation of SIRT1 Reduces Cellular Senescence through a SIRT1-FOXO1-Autophagy Axis.Biomolecules & therapeutics · 2017Article
- Lowering relative humidity level increases epidermal protein deimination and drives human filaggrin breakdown.Journal of dermatological science · 2017Article
- Alterations in Epidermal Eicosanoid Metabolism Contribute to Inflammation and Impaired Late Differentiation in FLG-Mutated Atopic Dermatitis.The Journal of investigative dermatology · 2017Article
- Induced Pluripotent Stem Cell Differentiation and Three-Dimensional Tissue Formation Attenuate Clonal Epigenetic Differences in Trichohyalin.Stem cells and development · 2016Article
- Endoplasmic Reticulum Calcium Regulates Epidermal Barrier Response and Desmosomal Structure.The Journal of investigative dermatology · 2016Review
- Towards a quantitative theory of epidermal calcium profile formation in unwounded skin.PloS one · 2015Article
- Skin barrier in atopic dermatitis: beyond filaggrin.Anais brasileiros de dermatologiaReview
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Authors and funding
12 authors.
Funding
Abstract
Multilayered human keratinocyte cultures increasingly are used to model human epidermis. Until now, studies utilizing human epidermal equivalents (HEEs) have been limited because previous preparations do not establish a normal epidermal permeability barrier. In this report, we show that reducing environmental humidity to 50% relative humidity yields HEEs that closely match human postnatal epidermis and have enhanced repair of the permeability barrier. These cultures display low transepidermal water loss and possess a calcium and pH gradient that resembles those seen in human epidermis. These cultures upregulate glucosylceramide synthase and make normal-appearing lipid lamellar bilayers. The epidermal permeability barrier of these cultures can be perturbed, using the identical tools previously described for human skin, and recover in the same time course seen during in vivo barrier recovery. These cultures will be useful for basic and applied studies on epidermal barrier function.
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