ArticleMolecular biology reports2014
DNA barcode ITS2 coupled with high resolution melting (HRM) analysis for taxonomic identification of Sideritis species growing in Greece.
Article in Molecular biology reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- DNA Barcoding and Comparative Chloroplast Marker Performance in Endemic Plants of Crete (Greece).Current issues in molecular biology · 2026Article
- Bioactive Compounds of Green Phenolic Extracts Obtained via Microwave-Assisted Extraction ofMolecules (Basel, Switzerland) · 2024Article
- Chloroplast mini-barcodes combined with high resolution melting analysis to identify herbal medicine Difengpi (Heliyon · 2024Article
- DNA Barcoding and Fertilization Strategies inInternational journal of molecular sciences · 2024Article
- Successful development of molecular diagnostic technology combining mini-barcoding and high-resolution melting for traditional Chinese medicine agarwood species based on single-nucleotide polymorphism in the chloroplast genome.Frontiers in plant science · 2024Article
- DNA barcoding in herbal medicine: Retrospective and prospective.Journal of pharmaceutical analysis · 2023Review
- Horizon scan of DNA-based methods for quality control and monitoring of herbal preparations.Frontiers in pharmacology · 2023Review
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- Cannabis Seeds Authentication by Chloroplast and Nuclear DNA Analysis Coupled with High-Resolution Melting Method for Quality Control Purposes.Cannabis and cannabinoid research · 2022Article
- Advancements and future prospective of DNA barcodes in the herbal drug industry.Frontiers in pharmacology · 2022Review
- Differentiation of Mitragyna speciosa, a narcotic plant, from allied Mitragyna species using DNA barcoding-high-resolution melting (Bar-HRM) analysis.Scientific reports · 2021Article
- A Comparison Study on Traditional Mixtures of Herbal Teas Used in Eastern Mediterranean Area.Frontiers in pharmacology · 2021Article
- Pitfalls and promises of raw drug identification techniques in the ayurvedic industry: an overview.3 Biotech · 2020Review
- Article
- Genus Sideritis, section Empedoclia in southeastern Europe and Turkey - studies in ethnopharmacology and recent progress of biological activities.Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences · 2019Review
- High throughput measure of diversity in cytoplasmic and nuclear traits for unravelling geographic distribution of rosemary.Ecology and evolution · 2019Article
- DNA Mini-Barcoding: A Derived Barcoding Method for Herbal Molecular Identification.Frontiers in plant science · 2019Review
- Multiuse of Bar-HRM for Ophiocordyceps sinensis identification and authentication.Scientific reports · 2018Article
- Authenticity Testing and Detection ofGenes · 2018Article
- Candidate DNA Barcode Tags Combined With High Resolution Melting (Bar-HRM) Curve Analysis for Authentication ofFrontiers in plant science · 2018Article
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Authors and funding
6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Identification of genotypes in Sideritis is complicated owing to the morphological similarity and common occurrence of natural hybridisation within Sideritis species. Species- and genotype-specific DNA markers are very useful for plant identification, breeding and preservation programs. Herein, a real-time polymerase chain reaction (PCR) of ITS2 barcode region coupled with high resolution melting-curve (HRM) analysis was evaluated for an accurate, rapid and sensitive tool for species identification focusing on seven Sideritis species growing in Greece. The HRM assay developed in this study is a rapid and straightforward method for the identification and discrimination of the investigated Sideritis species. This assay is simple compared to other genotyping methods as it does not require DNA sequencing or post-PCR processing. Therefore, this method offers a new alternative for rapid detection of Sideritis species.
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