ArticleThe Analyst2014
Matrix effects in biological mass spectrometry imaging: identification and compensation.
Article in The Analyst, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed.
- Computational Mass Spectrometry Imaging in the Era of AI.Chemical reviews · 2026Review
- Quantitative MALDI-MSI Workflow for Lipids with a LC-MS-Based Quality Control Pipeline.ACS measurement science au · 2026Article
- Towards spatial lipid profiling by using mass spectrometry: analytical challenges and applications.Analytical and bioanalytical chemistry · 2026Review
- Statistical Principles Define an Open-Source Differential Analysis Workflow for Mass Spectrometry Imaging Experiments with Complex Designs.bioRxiv : the preprint server for biology · 2026Article
- Imaging bioactive lipid isomers in acetaminophen-induced liver injury using nano-DESI tandem MS.Journal of lipid research · 2026Article
- Quantitative Mass Spectrometry Imaging of Intact Proteins in Tissues.Analytical chemistry · 2026Article
- Ion Mobility-Mass Spectrometry Imaging: Advances in Biomedical Research.Biotech (Basel (Switzerland)) · 2025Review
- Spatial quantitative metabolomics enables identification of remote and sustained ipsilateral cortical metabolic reprogramming after stroke.Nature metabolism · 2025Article
- Standard Addition as a Method for Quantitative Mass Spectrometry Imaging.Analytical chemistry · 2025Article
- Quantitative ambient mass spectrometry imaging in plants: A perspective on challenges and future applications.Current opinion in plant biology · 2025Review
- Single-cell mass spectrometry imaging of lipids in HeLa cells via tapping-mode scanning probe electrospray ionization.Communications chemistry · 2025Article
- Quantitative sampling by IR-MALDESI is not susceptible to tissue heterogeneity in a multi-organ model.Analytical and bioanalytical chemistry · 2025Article
- Nanospray Desorption Electrospray Ionization Mass Spectrometry Imaging (nano-DESI MSI): A Tutorial Review.ACS measurement science au · 2024Review
- Mass Spectrometry Advancements and Applications for Biomarker Discovery, Diagnostic Innovations, and Personalized Medicine.International journal of molecular sciences · 2024Review
- An Analytical Evaluation of Tools for Lipid Isomer Differentiation in Imaging Mass Spectrometry.International journal of mass spectrometry · 2024Article
- Quantitative mass spectrometry imaging (qMSI): A tutorial.Journal of mass spectrometry : JMS · 2024Article
- MALDI-MSI-LC-MS/MS Workflow for Single-Section Single Step Combined Proteomics and Quantitative Lipidomics.Analytical chemistry · 2024Article
- Quantitative mass spectrometry imaging: therapeutics & biomolecules.Chemical communications (Cambridge, England) · 2024Review
- Toward Omics-Scale Quantitative Mass Spectrometry Imaging of Lipids in Brain Tissue Using a Multiclass Internal Standard Mixture.Analytical chemistry · 2023Article
- Separation of Isobaric Lipids in Imaging Mass Spectrometry Using Gas-Phase Charge Inversion Ion/Ion Reactions.Journal of the American Society for Mass Spectrometry · 2023Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Matrix effects in mass spectrometry imaging (MSI) may affect the observed molecular distribution in chemical and biological systems. In this study, we use mouse brain tissue of a middle cerebral artery occlusion (MCAO) stroke model to examine matrix effects in nanospray desorption electrospray ionization MSI (nano-DESI MSI). This is achieved by normalizing the intensity of the sodium and potassium adducts of endogenous phosphatidylcholine (PC) species to the intensity of the corresponding adduct of the PC standard supplied at a constant rate with the nano-DESI solvent. The use of MCAO model with an ischemic region localized to one hemisphere of the brain enables immediate comparison of matrix effects within one ion image. Furthermore, significant differences in sodium and potassium concentrations in the ischemic region in comparison with the healthy tissue allowed us to distinguish between two types of matrix effects. Specifically, we discuss matrix effects originating from variations in alkali metal concentrations and matrix effects originating from variations in the molecular composition of the tissue. Compensation for both types of matrix effects was achieved by normalizing the signals corresponding to endogenous PC to the signals of the standards. This approach, which does not introduce any complexity in sample preparation, efficiently compensates for signal variations resulting from differences in the local concentrations of sodium and potassium in tissue sections and from the complexity of the extracted analyte mixture derived from local variations in molecular composition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.