ArticleMolecular biotechnology2014
Retransformation of marker-free potato for enhanced resistance against fungal pathogens by pyramiding chitinase and wasabi defensin genes.
Article in Molecular biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 24 citations in OpenAlex.
- Article
- Review
- Conventional gene stacking as a strategy to improve chickpea resistance to Ascochyta blight.Frontiers in plant science · 2025Article
- Pyramiding the antimicrobial PR1aCB and AATCB genes in 'Tarocco' blood orange (Citrus sinensis Osbeck) to enhance citrus canker resistance.Transgenic research · 2021Article
- Genetic Engineering Approaches for Enhanced Insect Pest Resistance in Sugarcane.Molecular biotechnology · 2021Review
- Transgene Stacking as Effective Tool for Enhanced Disease Resistance in Plants.Molecular biotechnology · 2020Review
- Plant defensins: types, mechanism of action and prospects of genetic engineering for enhanced disease resistance in plants.3 Biotech · 2019Review
- Enhanced resistance to Sclerotinia sclerotiorum in Brassica napus by co-expression of defensin and chimeric chitinase genes.Journal of applied genetics · 2016Article
- Article
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8 authors at 2 institutions in 4 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Multi-auto-transformation vector system has been one of the strategies to produce marker-free transgenic plants without using selective chemicals and plant growth regulators and thus facilitating transgene stacking. In the study reported here, retransformation was carried out in marker-free transgenic potato CV. May Queen containing ChiC gene (isolated from Streptomyces griseus strain HUT 6037) with wasabi defensin (WD) gene (isolated from Wasabia japonica) to pyramid the two disease resistant genes. Molecular analyses of the developed shoots confirmed the existence of both the genes of interest (ChiC and WD) in transgenic plants. Co-expression of the genes was confirmed by RT-PCR, northern blot, and western blot analyses. Disease resistance assay of in vitro plants showed that the transgenic lines co-expressing both the ChiC and WD genes had higher resistance against the fungal pathogens, Fusarium oxysporum (Fusarium wilt) and Alternaria solani (early blight) compared to the non-transformed control and the transgenic lines expressing either of the ChiC or WD genes. The disease resistance potential of the transgenic plants could be increased by transgene stacking or multiple transformations.
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