ArticlePloS one2014
Tualang honey improves human corneal epithelial progenitor cell migration and cellular resistance to oxidative stress in vitro.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 26 citations in OpenAlex.
- Medicinal activities of Tualang honey: a systematic review.BMC complementary medicine and therapies · 2024Pooled it
- Extracellular Vesicle-like Associated microRNAs in Monofloral Honeys: Molecular Characterization and Functional Pathways.International journal of molecular sciences · 2026Article
- Polyphenols in bee products and prevention of cell senescence.BioMedicine · 2024Review
- Network Pharmacology and Molecular Docking Analysis of Active Compounds in Tualang Honey against Atherosclerosis.Foods (Basel, Switzerland) · 2023Article
- Physicochemical Characteristics and Bioactive Compounds of Different Types of Honey and Their Biological and Therapeutic Properties: A Comprehensive Review.Antibiotics (Basel, Switzerland) · 2023Review
- The stingless bee honey protects against hydrogen peroxide-induced oxidative damage and lipopolysaccharide-induced inflammation in vitro.Saudi journal of biological sciences · 2021Article
- Physicochemical and Medicinal Properties of Tualang, Gelam and Kelulut Honeys: A Comprehensive Review.Nutrients · 2021Review
- Honey-incorporated nanofibre reduces replicative senescence of umbilical cord-derived mesenchymal stem cells.IET nanobiotechnology · 2020Article
- Tualang Honey Ameliorates Hypoxia-induced Memory Deficits by Reducing Neuronal Damage in the Hippocampus of Adult Male Sprague Dawley Rats.Turkish journal of pharmaceutical sciences · 2020Article
- Biocompatible Nutmeg Oil-Loaded Nanoemulsion as Phyto-Repellent.Frontiers in pharmacology · 2020Article
- Treatment of Corneal Alkali Burn with Chestnut Honey, Royal Jelly, and Chestnut Honey-Royal Jelly Mixture.Beyoglu eye journal · 2019Article
- Malaysian Tualang Honey Inhibits Hydrogen Peroxide-Induced Endothelial Hyperpermeability.Oxidative medicine and cellular longevity · 2019Article
- The anti-cancer effects of Tualang honey in modulating breast carcinogenesis: an experimental animal study.BMC complementary and alternative medicine · 2017Article
- covR Mediated Antibiofilm Activity of 3-Furancarboxaldehyde Increases the Virulence of Group A Streptococcus.PloS one · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stem cells with enhanced resistance to oxidative stress after in vitro expansion have been shown to have improved engraftment and regenerative capacities. Such cells can be generated by preconditioning them with exposure to an antioxidant. In this study we evaluated the effects of Tualang honey (TH), an antioxidant-containing honey, on human corneal epithelial progenitor (HCEP) cells in culture. Cytotoxicity, gene expression, migration, and cellular resistance to oxidative stress were evaluated. Immunofluorescence staining revealed that HCEP cells were holoclonal and expressed epithelial stem cell marker p63 without corneal cytokeratin 3. Cell viability remained unchanged after cells were cultured with 0.004, 0.04, and 0.4% TH in the medium, but it was significantly reduced when the concentration was increased to 3.33%. Cell migration, tested using scratch migration assay, was significantly enhanced when cells were cultured with TH at 0.04% and 0.4%. We also found that TH has hydrogen peroxide (H2O2) scavenging ability, although a trace level of H2O2 was detected in the honey in its native form. Preconditioning HCEP cells with 0.4% TH for 48 h showed better survival following H2O2-induced oxidative stress at 50 µM than untreated group, with a significantly lower number of dead cells (15.3 ± 0.4%) were observed compared to the untreated population (20.5 ± 0.9%, p<0.01). Both TH and ascorbic acid improved HCEP viability following induction of 100 µM H2O2, but the benefit was greater with TH treatment than with ascorbic acid. However, no significant advantage was demonstrated using 5-hydroxymethyl-2-furancarboxaldehyde, a compound that was found abundant in TH using GC/MS analysis. This suggests that the cellular anti-oxidative capacity in HCEP cells was augmented by native TH and was attributed to its antioxidant properties. In conclusion, TH possesses antioxidant properties and can improve cell migration and cellular resistance to oxidative stress in HCEP cells in vitro.
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