ArticlePloS one2014
Hsc70 contributes to cancer cell survival by preventing Rab1A degradation under stress conditions.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.
- Pooled analysis of prognostic value and clinical significance of Rab1A expression in human solid tumors.Medicine · 2019Pooled it
- Pristimerin Promotes Ubiquitination of HSPA8 and Activates the VAV1/ERK Pathway to Suppress TNBC Proliferation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- C118P Suppresses Gastric Cancer Growth via Promoting Autophagy-Lysosomal Degradation of RAB1A.Pharmaceutics · 2024Article
- Heat shock protein family A member 8 serving as a co-activator of transcriptional factor ETV4 up-regulates PHLDA2 to promote the growth of liver cancer.Acta pharmacologica Sinica · 2023Article
- Prognosis value and positive association of Rab1A/IL4Rα aberrant expression in gastric cancer.Scientific reports · 2023Article
- Review
- HSP70 and HSP90 in Cancer: Cytosolic, Endoplasmic Reticulum and Mitochondrial Chaperones of Tumorigenesis.Frontiers in oncology · 2022Review
- Review
- A novel circRNA-miRNA-mRNA network reveals hsa-circ-0040039 as a biomarker for intervertebral disc degeneration.The Journal of international medical research · 2021Article
- Similarities and Differences of Hsp70, hsc70, Grp78 and Mortalin as Cancer Biomarkers and Drug Targets.Cells · 2021Review
- Clinical value and potential association of Rab1A and FoxM1 aberrant expression in colorectal cancer.Scientific reports · 2020Article
- Expression of Rab1A in bladder cancer and its clinical implications.Experimental and therapeutic medicine · 2020Article
- Chaperone Mediated Autophagy Substrates and Components in Cancer.Frontiers in oncology · 2020Review
- miR‑139‑3p suppresses the invasion and migration properties of breast cancer cells by targeting RAB1A.Oncology reports · 2019Article
- Expression analysis and implication of Rab1A in gastrointestinal relevant tumor.Scientific reports · 2019Article
- Rab1A promotes proliferation and migration abilities via regulation of the HER2/AKT-independent mTOR/S6K1 pathway in colorectal cancer.Oncology reports · 2019Article
- Hsc70 Interacts with β4GalT5 to Regulate the Growth of Gliomas.Neuromolecular medicine · 2019Article
- Comprehensive transcriptomic analysis of heat shock proteins in the molecular subtypes of human breast cancer.BMC cancer · 2018Article
- Endogenous epitope tagging of heat shock protein 70 isoform Hsc70 using CRISPR/Cas9.Cell stress & chaperones · 2018Article
- Heat shock protein 70 inhibitors suppress androgen receptor expression in LNCaP95 prostate cancer cells.Cancer science · 2017Article
Corrections and comments
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Authors and funding
15 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heat shock cognate protein 70 (Hsc70) acts as a molecular chaperone for the maintenance of intracellular proteins, which allows cancer cells to survive under proteotoxic stress. We attempted to use Hsc70 to identify key molecules in cancer cell survival. Here, we performed mass-spectrometry-based proteomics analysis utilizing affinity purification with anti-Hsc70 antibodies; as a result, 83 differentially expressed proteins were identified under stress conditions. This result implies that there was a change in the proteins with which Hsc70 interacted in response to stress. Among the proteins identified under both serum-depleted and 5-fluorouracil-treated conditions, Rab1A was identified as an essential molecule for cancer cell survival. Hsc70 interacted with Rab1A in a chaperone-dependent manner. In addition, Hsc70 knockdown decreased the level of Rab1A and increased the level of its ubiquitination under stress conditions, suggesting that Hsc70 prevented the degradation of Rab1A denatured by stress exposure. We also found that Rab1A knockdown induced cell death by inhibition of autophagosome formation. Rab1A may therefore contribute to overcoming proteotoxic insults, which allows cancer cells to survive under stress conditions. Analysis of Hsc70 interactors provided insight into changes of intracellular status. We expect further study of the Hsc70 interactome to provide a more comprehensive understanding of cancer cell physiology.
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