ArticleStem cells (Dayton, Ohio)2014
Engineering the human thymic microenvironment to support thymopoiesis in vivo.
Article in Stem cells (Dayton, Ohio), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 57 citations in OpenAlex.
- Generation of Functional Patient-Specific Thymus Organoids From Human Pluripotent Stem Cells (hPSCs) Using Air-Liquid Interface Culture.Bio-protocol · 2026Article
- A Thymus-Independent Artificial Organoid System Supports Complete Thymopoiesis from Rhesus Macaque-Derived Hematopoietic Stem and Progenitor Cells.Biomedicines · 2025Article
- A thymus-independent artificial organoid system supports complete thymopoiesis from Rhesus macaque-derived hematopoietic stem and progenitor cells.bioRxiv : the preprint server for biology · 2025Article
- Development of Thymic Organoids Heterotopically to Educate and Induce T Lymphocytes.Immunity, inflammation and disease · 2025Article
- Generation of functional thymic organoids from human pluripotent stem cells.Stem cell reports · 2023Article
- Investigating Thymic Epithelial Cell Diversity Using Systems Biology.Journal of immunology (Baltimore, Md. : 1950) · 2023Review
- Review
- The potential role of the thymus in immunotherapies for acute myeloid leukemia.Frontiers in immunology · 2023Review
- Engineered T cells from induced pluripotent stem cells: from research towards clinical implementation.Frontiers in immunology · 2023Review
- Inborn errors of immunity associated with defects of thymic development.Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology · 2022Review
- Progress on methods of T lymphocyte developmentZhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2022Review
- Thymic Extracellular Matrix in the Thymopoiesis: Just a Supporting?Biotech (Basel (Switzerland)) · 2022Article
- Thymus Functionality Needs More Than a Few TECs.Frontiers in immunology · 2022Review
- Recent Advancements in Regenerative Approaches for Thymus Rejuvenation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- T cell regeneration after immunological injury.Nature reviews. Immunology · 2021Review
- Advances in Adoptive Cell Therapy Using Induced Pluripotent Stem Cell-Derived T Cells.Frontiers in immunology · 2021Review
- Harnessing Mesenchymal Stromal Cells for the Engineering of Human Hematopoietic Niches.Frontiers in immunology · 2021Review
- Premature Senescence and Increased Oxidative Stress in the Thymus of Down Syndrome Patients.Frontiers in immunology · 2021Article
- Reconstitution of a functional human thymus by postnatal stromal progenitor cells and natural whole-organ scaffolds.Nature communications · 2020Article
- Enabling allogeneic therapies: CIRM-funded strategies for immune tolerance and immune evasion.Stem cells translational medicine · 2020Review
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
A system that allows manipulation of the human thymic microenvironment is needed both to elucidate the extrinsic mechanisms that control human thymopoiesis and to develop potential cell therapies for thymic insufficiency. In this report, we developed an implantable thymic microenvironment composed of two human thymic stroma populations critical for thymopoiesis; thymic epithelial cells (TECs) and thymic mesenchyme (TM). TECs and TM from postnatal human thymi were cultured in specific conditions, allowing cell expansion and manipulation of gene expression, before reaggregation into a functional thymic unit. Human CD34+ hematopoietic stem and progenitor cells (HSPC) differentiated into T cells in the aggregates in vitro and in vivo following inguinal implantation of aggregates in immune deficient mice. Cord blood HSPC previously engrafted into murine bone marrow (BM), migrated to implants, and differentiated into human T cells with a broad T cell receptor repertoire. Furthermore, lentiviral-mediated expression of vascular endothelial growth factor in TM enhanced implant size and function and significantly increased thymocyte production. These results demonstrate an in vivo system for the generation of T cells from human HSPC and represent the first model to allow manipulation of gene expression and cell composition in the microenvironment of the human thymus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.