ReviewJournal of immunological methods2014
Development and implementation of a proficiency testing program for Luminex bead-based cytokine assays.
Review in Journal of immunological methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed.
- Identification of multiple arbovirus infections/exposure in Northeastern Brazil using a multiplex microsphere immunoassay.Journal of clinical microbiology · 2026Article
- Review
- High BMP7 Expression May Worsen Airway Disease in COPD by Altering Epithelial Cell Behavior.International journal of chronic obstructive pulmonary disease · 2025Article
- Sources of variability in Luminex bead-based cytokine assays: Evidence from twelve years of multi-site proficiency testing.Journal of immunological methods · 2024Article
- Immunoassays and Mass Spectrometry for Determination of Protein Concentrations in Genetically Modified Crops.Journal of agricultural and food chemistry · 2024Review
- Maternal immunity shapes biomarkers of germinal center development in HIV-exposed uninfected infants.Frontiers in immunology · 2024Article
- Redefining serological diagnostics with immunoaffinity proteomics.Clinical proteomics · 2023Review
- The Cytokine Profile in Different Stages of Schistosomiasis Japonica.Pathogens (Basel, Switzerland) · 2023Article
- Expression Pattern of Cytokines in Patients with Chronic Hepatitis B Receiving PEGinterferon Therapy.International journal of general medicine · 2023Article
- Dynamic immune and exosome transcriptomic responses in patients undergoing psychostimulant methamphetamine withdrawal.Frontiers in cellular neuroscience · 2022Article
- Variation of Immune Cell Responses in Humans Reveals Sex-Specific Coordinated Signaling Across Cell Types.Frontiers in immunology · 2022Article
- An evaluation of the recognised systemic inflammatory biomarkers of chronic sub-optimal inflammation provides evidence for inflammageing (IFA) during multiple sclerosis (MS).Immunity & ageing : I & A · 2021Review
- Association of a Network of Immunologic Response and Clinical Features With the Functional Recovery From Crotalinae Snakebite Envenoming.Frontiers in immunology · 2021Article
- Biomarker profile in stable Fontan patients.International journal of cardiology · 2020Article
- A high-throughput and multiplex microsphere immunoassay based on non-structural protein 1 can discriminate three flavivirus infections.PLoS neglected tropical diseases · 2019Article
- Eotaxin‑1 and MCP‑1 serve as circulating indicators in response to power frequency electromagnetic field exposure in mice.Molecular medicine reports · 2018Article
- Optimization and qualification of an Fc Array assay for assessments of antibodies against HIV-1/SIV.Journal of immunological methods · 2018Article
- Simultaneous Detection of Nine Key Bacterial Respiratory Pathogens Using Luminex xTAGInternational journal of environmental research and public health · 2017Article
- Psychiatric disorders biochemical pathways unraveled by human brain proteomics.European archives of psychiatry and clinical neuroscience · 2017Review
- Identifying baseline immune-related biomarkers to predict clinical outcome of immunotherapy.Journal for immunotherapy of cancer · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Luminex bead array assays are widely used for rapid biomarker quantification due to the ability to measure up to 100 unique analytes in a single well of a 96-well plate. There has been, however, no comprehensive analysis of variables impacting assay performance, nor development of a standardized proficiency testing program for laboratories performing these assays. To meet this need, the NIH/NIAID and the Cancer Immunotherapy Consortium of the Cancer Research Institute collaborated to develop and implement a Luminex assay proficiency testing program as part of the NIH/NIAID-sponsored External Quality Assurance Program Oversight Laboratory (EQAPOL) at Duke University. The program currently monitors 25 domestic and international sites with two external proficiency panels per year. Each panel includes a de-identified commercial Luminex assay kit with standards to quantify human IFNγ, TNFα, IL-6, IL-10 and IL-2, and a series of recombinant cytokine-spiked human serum samples. All aspects of panel development, testing and shipping are performed under GCLP by EQAPOL support teams. Following development testing, a comprehensive site proficiency scoring system comprised of timeliness, protocol adherence, accuracy and precision was implemented. The overall mean proficiency score across three rounds of testing has remained stable (EP3: 76%, EP4: 75%, EP5: 77%); however, a more detailed analysis of site reported results indicates a significant improvement of intra- (within) and inter- (between) site variation, suggesting that training and remediation for poor performing sites may be having a positive impact on proficiency. Through continued proficiency testing, identification of variables affecting Luminex assay outcomes will strengthen efforts to bring standardization to the field.
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Registered trials
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