ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2014
Comparison and optimization of two MALDI-TOF MS platforms for the identification of medically relevant yeast species.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Performance and workflow comparison of the VITEK MS PRIME and Bruker Biotyper MALDI-TOF MS systems.Journal of clinical microbiology · 2025Article
- Spectral Precision: Recent Advances in Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for Pathogen Detection and Resistance Profiling.Microorganisms · 2025Review
- Biology and genetic diversity ofVirulence · 2024Article
- Application of MALDI-TOF MS for enumerating bacterial constituents of defined consortia.Applied microbiology and biotechnology · 2023Article
- MALDI-TOF Mass Spectrometry in Clinical Analysis and Research.ACS measurement science au · 2022Review
- Mass spectrometry in research laboratories and clinical diagnostic: a new era in medical mycology.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Review
- An analysis of the population of Cryptococcus neoformans strains isolated from animals in Poland, in the years 2015-2019.Scientific reports · 2021Article
- Evaluation of three sample preparation methods for the identification of clinical strains by using two MALDI-TOF MS systems.Journal of mass spectrometry : JMS · 2021Article
- Rapid microbial identification and colistin resistance detection via MALDI-TOF MS using a novel on-target extraction of membrane lipids.Scientific reports · 2020Article
- Recent applications of mass spectrometry in bacterial lipidomics.Analytical and bioanalytical chemistry · 2020Review
- Adaption of the Aristotle Classifier for Accurately Identifying Highly Similar Bacteria Analyzed by MALDI-TOF MS.Analytical chemistry · 2020Article
- Model-Based Spectral Library Approach for Bacterial Identification via Membrane Glycolipids.Analytical chemistry · 2019Article
- Evaluation of a new matrix-assisted laser desorption/ionization time-of-flight mass spectrometry system for the identification of yeast isolation.Journal of clinical laboratory analysis · 2019Article
- A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification.Journal of fungi (Basel, Switzerland) · 2019Review
- Pathogen Identification Direct From Polymicrobial Specimens Using Membrane Glycolipids.Scientific reports · 2018Article
- Performance of Microflex LT Biotyper and VITEK MS for Routine Identification of Yeasts.Annals of laboratory medicine · 2018Article
- Rapid one-step extraction method for the identification of molds using MALDI-TOF MS.Diagnostic microbiology and infectious disease · 2018Article
- Taxonomy and evolution ofComputational and structural biotechnology journal · 2018Review
- Identification of the ESKAPE pathogens by mass spectrometric analysis of microbial membrane glycolipids.Scientific reports · 2017Article
- Ventilator-Associated Pneumonia: The Role of Emerging Diagnostic Technologies.Seminars in respiratory and critical care medicine · 2017Review
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4 authors.
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Abstract
The rapid identification of yeast is essential for the optimization of antifungal therapy. The objective of our study was to evaluate two matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) platforms, the bioMérieux VITEK MS (IVD Knowledgebase v.2.0) and Bruker Biotyper (software version 3.1), for the rapid identification of medically relevant yeast. One hundred and seventeen isolates, representing six genera and 18 species, were analyzed using multiple direct smear methods to optimize identification. Sequence analysis was the gold standard for comparison. Isolates were analyzed with VITEK MS using the direct smear method +/- a 25 % formic acid on-plate extraction. For Biotyper, isolates were analyzed using direct smear without formic acid, and with 25 % and 100 % formic acid on-plate extractions. When all methods were included, VITEK MS correctly identified 113 (96.6 %) isolates after 24 h with one misidentification, and Biotyper correctly identified 77 (65.8 %) isolates using a threshold of ≥2.0 with no misidentifications. Using a revised threshold of ≥1.7, Biotyper correctly identified 103 (88.0 %) isolates, with 3 (2.6 %) misidentifications. For both platforms, the number of identifications was significantly increased using a formic acid overlay (VITEK MS, p < 0.01; Biotyper, p < 0.001), and reducing the Biotyper threshold from ≥2.0 to ≥1.7 significantly increased the rate of identification (p < 0.001). The data in this study demonstrate that the direct smear method with on-plate formic acid extraction can be used for yeast identification on both MS platforms, and more isolates are identified using the VITEK MS system (p < 0.01).
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