ArticleAdvanced biomedical research2014
Comparative evaluation of three different methods for HbA1c measurement with High-performance liquid chromatography in diabetic patients.
Article in Advanced biomedical research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Comparative analysis of enzymatic assay for HbA1c measurement with high performance liquid chromatography.Scientific reports · 2026Article
- A Novel Aberrant HbF Peak with Electrophoretic Shift in A1c of a Patient with Chronic Lymphocytic Leukemia (CLL) Was Reversible to Give Interpretable Results.Biomedicines · 2026Article
- Association of serum adiponectin level with glycemic control and atherogenic lipid profile in Sudanese patients with type 2 diabetes mellitus.BMC endocrine disorders · 2025Article
- Real-world smartphone-based point-of-care diagnostics in primary health care to monitor HbA1c levels in people with diabetes.Communications medicine · 2025Article
- Clinical Risk Assessment and Comparison of Bias between Laboratory Methods for Estimation of HbA1c for Glycated Hemoglobin in Hyperglycemic Patients.Current diabetes reviews · 2024Article
- Establishment of reference interval for hemoglobin A1C and other hemoglobin subfractions for healthy Saudi adults.PloS one · 2024Article
- Cases of fulminant type 1 and type 2 diabetes mellitus whose HbA1c levels were unmeasurable due to increased labile HbA1c.Diabetology international · 2022Article
- Helicobacter pylori infection is not an independent risk factor of non-alcoholic fatty liver disease in China.BMC gastroenterology · 2022Article
- Article
- Changes in hemoglobin profile reflect autologous blood transfusion misuse in sports.Internal and emergency medicine · 2018Article
- Magnetite-Quantum Dot Immunoarray for Plasmon-Coupled-Fluorescence Imaging of Blood Insulin and Glycated Hemoglobin.ACS sensors · 2017Article
- Association of lipocalin-2 level, glycemic status and obesity in type 2 diabetes mellitus.BMC research notes · 2017Article
- Significance of HbA1c Test in Diagnosis and Prognosis of Diabetic Patients.Biomarker insights · 2016Review
- Effect of the different assays of HbA1c on diabetic patients monitoring.Journal of diabetes and metabolic disorders · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe global prevalence of diabetes mellitus is increasing rapidly. Measurement of glycated hemoglobin, predominantly HbA1c, is fundamental to the management of patients with diabetes. HbA1c is used to monitor long-term glycemic control, adjust therapy, assess the quality of diabetes care and predict the risk for the development of complications. While HbA1c is the standard method for long-term glycemic control in diabetic patients, there are different methods for measurement of HbA1c and all laboratories do not use the reference method (high-performance liquid chromatography [HPLC]). The objective of this study is comparison of three different methods with HPLC to find out which method has an acceptable concordance and correlation with the reference method. MATERIALS AND
methodsFifty-eight diabetic patients were assessed in this study. The blood sample of each patient was checked with Diazyme (enzymatic assay), Nycocard (boronate-affinity binding) and Biosystem (micro column chromatography). The values of HbA1c of each method were compared with the Knauer-HPLC results.
resultsTHE MEANS OF THE DIFFERENTIAL VALUES BETWEEN EACH METHOD AND HPLC IN THE ANOVA TEST ARE AS FOLLOWS: M = 1.8, SD = 1.09 for Nycocard-HPLC; M = 1.5, SD = 1.08 for biosystem-HPLC; M = 1.3, SD = 1.2 for Diazyme-HPLC. Pearson's correlation coefficient between HPLC and Nycocard; 0.76, HPLC and Diazyme; 0.75 and between HPLC and Biosystem was 0.68. Linear regression parameters for each method with HPLC were also determined.
conclusionDiazyme had a better performance and showed a greater concordance with HPLC among others, although it was not an ideal alternative for HPLC.
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