ArticleIranian journal of reproductive medicine2014
Effects of ascorbic acid on sperm motility, viability, acrosome reaction and DNA integrity in teratozoospermic samples.
Article in Iranian journal of reproductive medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 54 citations in OpenAlex.
- Comparison of the Effect of Ceratonia siliqua L. (Carob) Syrup and Vitamin E on Sperm Parameters, Oxidative Stress Index, and Sex Hormones in Infertile Men: a Randomized Controlled Trial.Reproductive sciences (Thousand Oaks, Calif.) · 2021Trial
- Improvement of Mouse Spermatozoa Freezing at -80°C With Ascorbic Acid 2-Glucoside at Thawing Phase.Andrology · 2026Article
- Canthaxanthin enhances the quality of cryopreserved goat spermatozoa.Veterinary research forum : an international quarterly journal · 2026Article
- Pharmacological and toxicological insights into the ayurvedic formulation Rasasindura.Scientific reports · 2025Article
- Effect of glutathione, ascorbic acid, and their combination on the quality and fertility of cryopreserved spermatozoa of Black Bengal buck.Veterinary and animal science · 2025Article
- Evaluation of ascorbic acid as an intervention of metal toxicity in dogs in Kabwe district.Veterinary and animal science · 2025Article
- Ascorbic acid 2-glucoside improves survival, quality, and fertility of frozen-thawed C57Bl/6J and C57Bl/6N mouse spermatozoa.Andrology · 2025Article
- Role of oxidative stress in male infertility.Reproduction & fertility · 2023Review
- Human Sperm as an In Vitro Model to Assess the Efficacy of Antioxidant Supplements during Sperm Handling: A Narrative Review.Antioxidants (Basel, Switzerland) · 2023Review
- Reproductive toxicity of combined effects of endocrine disruptors on human reproduction.Frontiers in cell and developmental biology · 2023Review
- In Vitro Combination of Ascorbic and Ellagic Acids in Sperm Oxidative Damage Inhibition.International journal of molecular sciences · 2022Article
- Toxicity assessment of chronic exposure to common insecticides and bee medications on colony development and drones sperm parameters.Ecotoxicology (London, England) · 2021Article
- Oxidative Stress and BPA Toxicity: An Antioxidant Approach for Male and Female Reproductive Dysfunction.Antioxidants (Basel, Switzerland) · 2020Review
- Effects of Qilin pills on spermatogenesis, reproductive hormones, oxidative stress, and the TSSK2 gene in a rat model of oligoasthenospermia.BMC complementary medicine and therapies · 2020Article
- GSTO2 Isoforms Participate in the Oxidative Regulation of the Plasmalemma in Eutherian Spermatozoa during Capacitation.Antioxidants (Basel, Switzerland) · 2019Article
- Effect of vitamin D on apoptotic marker, reactive oxygen species and human sperm parameters during the process of cryopreservation.Iranian journal of basic medical sciences · 2019Article
- [Association of advanced oxidation protein products in seminal plasma with teratospermia and outcome parameters ofNan fang yi ke da xue xue bao = Journal of Southern Medical University · 2019Article
- Effect of antioxidants on BPA-induced stress on sperm function in a mouse model.Scientific reports · 2019Article
- Basic and clinical genetic studies on male infertility in Iran during 2000-2016: A review.International journal of reproductive biomedicine · 2018Review
- Reproductive toxicity after levetiracetam administration in male rats: Evidence for role of hormonal status and oxidative stress.PloS one · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOxidative stress in teratozoospermic semen samples caused poor assisted reproductive techniques (ART) outcomes. Among antioxidants, ascorbic acid is a naturally occurring free radical scavenger and as such its presence assists various other mechanisms in decreasing numerous disruptive free radical processes.
objectiveThe main goal of this study was to evaluate potential protective effects of ascorbic acid supplementation during in vitro culture of teratozoospermic specimens. MATERIALS AND
methodsTeratozoospermic semen samples that collected from 15 volunteers were processed, centrifuged and incubated at 37(o)C until sperm swimmed-up. Supernatant was divided into four groups and incubated at 37(o)C for one hour under different experimental conditions: Control, 10 µm A23187, 600µm ascorbic acid and 10 µm A23187+600 µm ascorbic acid. After incubation sperm motility, viability, acrosome reaction, DNA damage and malondialdehyde levels were evaluated.
resultsOur results indicated that after one hour incubation, ascorbic acid significantly reduced malondialdehyde level in ascorbic acid group (1.4±0.11 nmol/ml) compared to control group (1.58±0.13 nmol/ml) (p<0.001). At the end of incubation, progressive motility and viability in ascorbic acid group (64.5±8.8% and 80.3±6.4%, respectively) were significantly (p<0.05 and p<0.001, respectively) higher than the control group (54.5±6.8% and 70.9±7.3%, respectively). A23187 significantly (p<0.0001) increased acrosome reaction in A23187 group (37.3±5.6%) compared to control group (8.5±3.2%) and this effect of A23187 attenuated by ascorbic acid in ascorbic acid+A23187 group (17.2±4.4%). DNA fragmentation in ascorbic acid group (20±4.1%) was significantly (p<0.001) lower than controls (28.9±4.6%).
conclusionIn vitro ascorbic acid supplementation during teratozoospermic semen processing for ART could protect teratozoospermic specimens against oxidative stress, and it could improve ART outcome.
Indexed as
Identifiers
24799867PMC4009562W22324847What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.