ArticleDevelopmental neurobiology2014
Transcriptome of Atoh7 retinal progenitor cells identifies new Atoh7-dependent regulatory genes for retinal ganglion cell formation.
Article in Developmental neurobiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 42 citations in OpenAlex.
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- A Benzarone Derivative Inhibits EYA to Suppress Tumor Growth in SHH Medulloblastoma.Cancer research · 2024Article
- Identification of the CDH18 gene associated with age-related macular degeneration using weighted gene co-expression network analysis.Frontiers in genetics · 2024Article
- Comparative expression analysis of the Atoh7 gene regulatory network in the mouse and chicken auditory hindbrain.Cell and tissue research · 2023Article
- Signaling - transcription interactions in mouse retinal ganglion cells early axon pathfinding -a literature review.Frontiers in ophthalmology · 2023Review
- Pou4f1-Tbr1 transcriptional cascade controls the formation of Jam2-expressing retinal ganglion cells.Frontiers in ophthalmology · 2023Article
- Molecular profiling of stem cell-derived retinal pigment epithelial cell differentiation established for clinical translation.Stem cell reports · 2022Article
- Genetic control of retinal ganglion cell genesis.Cellular and molecular life sciences : CMLS · 2021Review
- Two new genetically modified mouse alleles labeling distinct phases of retinal ganglion cell development by fluorescent proteins.Developmental dynamics : an official publication of the American Association of Anatomists · 2020Article
- TheProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Transcriptome analysis of the zebrafishFASEB bioAdvances · 2020Article
- Neurogenesis and Specification of Retinal Ganglion Cells.International journal of molecular sciences · 2020Review
- On the Generation and Regeneration of Retinal Ganglion Cells.Frontiers in cell and developmental biology · 2020Article
- Wiring the Binocular Visual Pathways.International journal of molecular sciences · 2019Review
- Fate-restricted retinal progenitor cells adopt a molecular profile and spatial position distinct from multipotent progenitor cells.Developmental biology · 2018Article
- Essential roles of mitochondrial biogenesis regulator Nrf1 in retinal development and homeostasis.Molecular neurodegeneration · 2018Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The bHLH transcription factor ATOH7 (Math5) is essential for establishing retinal ganglion cell (RGC) fate. However, Atoh7-expressing retinal progenitor cells (RPCs) can give rise to all retinal cell types, suggesting that other factors are involved in specifying RGCs. The basis by which a subpopulation of Atoh7-expressing RPCs commits to an RGC fate remains uncertain but is of critical importance to retinal development since RGCs are the earliest cell type to differentiate. To better understand the regulatory mechanisms leading to cell-fate specification, a binary genetic system was generated to specifically label Atoh7-expressing cells with green fluorescent protein (GFP). Fluorescence-activated cell sorting (FACS)-purified GFP(+) and GFP(-) cells were profiled by RNA-seq. Here, we identify 1497 transcripts that were differentially expressed between the two RPC populations. Pathway analysis revealed diminished growth factor signaling in Atoh7-expressing RPCs, indicating that these cells had exited the cell cycle. In contrast, axon guidance signals were enriched, suggesting that axons of Atoh7-expressing RPCs were already making synaptic connections. Notably, many genes enriched in Atoh7-expressing RPCs encoded transcriptional regulators, and several were direct targets of ATOH7, including, and unexpectedly, Ebf3 and Eya2. We present evidence for a Pax6-Atoh7-Eya2 pathway that acts downstream of Atoh7 but upstream of differentiation factor Pou4f2. EYA2 is a protein phosphatase involved in protein-protein interactions and posttranslational regulation. These properties, along with Eya2 as an early target gene of ATOH7, suggest that EYA2 functions in RGC specification. Our results expand current knowledge of the regulatory networks operating in Atoh7-expressing RPCs and offer new directions for exploring the earliest aspects of retinogenesis.
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Registered trials
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