ArticleMolecular imaging and biology2014
Oatp1 enhances bioluminescence by acting as a plasma membrane transporter for D-luciferin.
Article in Molecular imaging and biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Review
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- Engineering water exchange is a safe and effective method for magnetic resonance imaging in diverse cell types.Journal of biological engineering · 2024Article
- Engineering water exchange is a safe and effective method for magnetic resonance imaging in diverse cell types.bioRxiv : the preprint server for biology · 2023Article
- Safe harbor-targeted CRISPR-Cas9 homology-independent targeted integration for multimodality reporter gene-based cell tracking.Science advances · 2021Article
- Organic anion-transporting polypeptide 1B3 as a dual reporter gene for fluorescence and magnetic resonance imaging.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Review
- Continuous long-term cytotoxicity monitoring in 3D spheroids of beetle luciferase-expressing hepatocytes by nondestructive bioluminescence measurement.BMC biotechnology · 2017Article
- Preclinical imaging methods for assessing the safety and efficacy of regenerative medicine therapies.NPJ Regenerative medicine · 2017Review
- In-depth Characterization of Firefly Luciferase as a Reporter of Circadian Gene Expression in Mammalian Cells.Journal of biological rhythms · 2016Article
- MRI Reporter Genes for Noninvasive Molecular Imaging.Molecules (Basel, Switzerland) · 2016Review
- Development of Timd2 as a reporter gene for MRI.Magnetic resonance in medicine · 2016Article
- Combining fluorescence and bioluminescence microscopy.Microscopy research and technique · 2015Article
- Molecular Imaging of Membrane Transporters' Activity in Cancer: a Picture is Worth a Thousand Tubes.The AAPS journal · 2015Review
- Beyond D-luciferin: expanding the scope of bioluminescence imaging in vivo.Current opinion in chemical biology · 2014Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
purposeBioluminescence imaging is a powerful tool for studying gene expression and cell migration in intact living organisms. However, production of bioluminescence by cells transfected to express luciferase can be limited by the rate of plasma membrane transport of its substrate D-luciferin. We sought to identify a plasma membrane transporter for D-luciferin that could be expressed alongside luciferase to increase transmembrane flux of its substrate and thereby increase light output. PROCEDURES: Luciferase-expressing cells were transfected with a lentivirus encoding the rat reno-hepatic organic anion transporter protein, Oatp1, which was identified as a potential transporter for D-luciferin. Light output was compared between cells expressing luciferase and those also expressing Oatp1.
resultsIn two cell lines and in mouse xenografts, co-expression of Oatp1 with luciferase increased light output by several fold, following addition of luciferin.
conclusionsThe increase in light output thus obtained will allow more sensitive detection of luciferase-expressing cells in vivo.
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