ArticleScientific reports2014
Assembly and validation of versatile transcription activator-like effector libraries.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 10 citations in OpenAlex.
- Reprogramming cell fate with artificial transcription factors.FEBS letters · 2018Review
- Reprogramming cell fate with a genome-scale library of artificial transcription factors.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Exploiting the CRISPR/Cas9 PAM Constraint for Single-Nucleotide Resolution Interventions.PloS one · 2016Article
- Reconfigurable hybrid interface for molecular marker diagnostics and in-situ reporting.Biosensors & bioelectronics · 2015Article
- RiboTALE: A modular, inducible system for accurate gene expression control.Scientific reports · 2015Article
- Controlling gene networks and cell fate with precision-targeted DNA-binding proteins and small-molecule-based genome readers.The Biochemical journal · 2014Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The ability to perturb individual genes in genome-wide experiments has been instrumental in unraveling cellular and disease properties. Here we introduce, describe the assembly, and demonstrate the use of comprehensive and versatile transcription activator-like effector (TALE) libraries. As a proof of principle, we built an 11-mer library that covers all possible combinations of the nucleotides that determine the TALE-DNA binding specificity. We demonstrate the versatility of the methodology by constructing a constraint library, customized to bind to a known p53 motif. To verify the functionality in assays, we applied the 11-mer library in yeast-one-hybrid screens to discover TALEs that activate human SCN9A and miR-34b respectively. Additionally, we performed a genome-wide screen using the complete 11-mer library to confirm known genes that confer cycloheximide resistance in yeast. Considering the highly modular nature of TALEs and the versatility and ease of constructing these libraries we envision broad implications for high-throughput genomic assays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.