ArticleThe Journal of biological chemistry2014
UVA light-excited kynurenines oxidize ascorbate and modify lens proteins through the formation of advanced glycation end products: implications for human lens aging and cataract formation.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 2 of them syntheses that pooled it.
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Who cites it
26 citing papers in PubMed, 2 syntheses or guidelines pooled it, 68 citations in OpenAlex.
- Correlation of Sunlight Exposure and Different Morphological Types of Age-Related Cataract.BioMed research international · 2021Pooled it
- Cataract frequency and subtypes involved in workers assessed for their solar radiation exposure: a systematic review.Acta ophthalmologica · 2018Pooled it
- Assessment of Kynurenine Pathway Enzyme Activity in Ocular Diseases: Associations with Cataract, Diabetes, Glaucoma, and Pseudoexfoliation Syndrome.Journal of clinical medicine · 2025Article
- [From ozone depletion to the lens: effects of climate change on cataract progression].Die Ophthalmologie · 2025Review
- UV light and the ocular lens: a review of exposure models and resulting biomolecular changes.Frontiers in ophthalmology · 2024Review
- Article
- Mitotic Activation Around Wound Edges and Epithelialization Repair in UVB-Induced Capsular Cataracts.Investigative ophthalmology & visual science · 2021Article
- Kinetic Studies of Antioxidant Properties of Ovothiol A.Antioxidants (Basel, Switzerland) · 2021Article
- Protein posttranslational modification (PTM) by glycation: Role in lens aging and age-related cataractogenesis.Experimental eye research · 2021Review
- Autofluorescence as a noninvasive biomarker of senescence and advanced glycation end products in Caenorhabditis elegans.NPJ aging and mechanisms of disease · 2021Article
- Protein Stability and Photostability under In Vitro Vitreal Conditions - Implications for Long Acting Delivery of Protein Therapeutics for Ocular Disease.Pharmaceutical research · 2020Article
- Occupational radiation exposure and excess additive risk of cataract incidence in a cohort of US radiologic technologists.Occupational and environmental medicine · 2020Article
- Phototoxicity of environmental radiations in human lens: revisiting the pathogenesis of UV-induced cataract.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2019Review
- A biologically based mathematical model for spontaneous and ionizing radiation cataractogenesis.PloS one · 2019Article
- Protective Effects of Lanosterol Synthase Up-Regulation in UV-B-Induced Oxidative Stress.Frontiers in pharmacology · 2019Article
- Occupational radiation exposure and risk of cataract incidence in a cohort of US radiologic technologists.European journal of epidemiology · 2018Article
- High-throughput sequencing reveals novel lincRNA in age-related cataract.International journal of molecular medicine · 2017Article
- UVA Light-mediated Ascorbate Oxidation in Human Lenses.Photochemistry and photobiology · 2017Article
- Small molecules, both dietary and endogenous, influence the onset of lens cataracts.Experimental eye research · 2017Review
- Identification of Kynoxazine, a Novel Fluorescent Product of the Reaction between 3-Hydroxykynurenine and Erythrulose in the Human Lens, and Its Role in Protein Modification.The Journal of biological chemistry · 2016Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Advanced glycation end products (AGEs) contribute to lens protein pigmentation and cross-linking during aging and cataract formation. In vitro experiments have shown that ascorbate (ASC) oxidation products can form AGEs in proteins. However, the mechanisms of ASC oxidation and AGE formation in the human lens are poorly understood. Kynurenines are tryptophan oxidation products produced from the indoleamine 2,3-dioxygenase (IDO)-mediated kynurenine pathway and are present in the human lens. This study investigated the ability of UVA light-excited kynurenines to photooxidize ASC and to form AGEs in lens proteins. UVA light-excited kynurenines in both free and protein-bound forms rapidly oxidized ASC, and such oxidation occurred even in the absence of oxygen. High levels of GSH inhibited but did not completely block ASC oxidation. Upon UVA irradiation, pigmented proteins from human cataractous lenses also oxidized ASC. When exposed to UVA light (320-400 nm, 100 milliwatts/cm(2), 45 min to 2 h), young human lenses (20-36 years), which contain high levels of free kynurenines, lost a significant portion of their ASC content and accumulated AGEs. A similar formation of AGEs was observed in UVA-irradiated lenses from human IDO/human sodium-dependent vitamin C transporter-2 mice, which contain high levels of kynurenines and ASC. Our data suggest that kynurenine-mediated ASC oxidation followed by AGE formation may be an important mechanism for lens aging and the development of senile cataracts in humans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.