ArticlePloS one2014
Functional analysis of BcBem1 and its interaction partners in Botrytis cinerea: impact on differentiation and virulence.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 27 citations in OpenAlex.
- Article
- Article
- Deciphering the Dynamics of Signaling Cascades and Virulence Factors ofMicroorganisms · 2021Article
- Tracking Fungal Growth: Establishment of Arp1 as a Marker for Polarity Establishment and Active Hyphal Growth in Filamentous Ascomycetes.Journal of fungi (Basel, Switzerland) · 2021Article
- Molecular basis of pathogenesis of postharvest pathogenic Fungi and control strategy in fruits: progress and prospect.Molecular horticulture · 2021Review
- Silencing of the Slt2-Type MAP KinaseInternational journal of molecular sciences · 2021Article
- Clathrin Is Important for Virulence Factors Delivery in the Necrotrophic FungusFrontiers in plant science · 2021Article
- The Destructive Fungal PathogenPathogens (Basel, Switzerland) · 2020Review
- A Similar Secretome Disturbance as a Hallmark of Non-pathogenicFrontiers in microbiology · 2019Article
- Update on Nox function, site of action and regulation inFungal biology and biotechnology · 2016Article
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5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
In phytopathogenic fungi the establishment and maintenance of polarity is not only essential for vegetative growth and differentiation, but also for penetration and colonization of host tissues. We investigated orthologs of members of the yeast polarity complex in the grey mould fungus Botrytis cinerea: the scaffold proteins Bem1 and Far1, the GEF (guanine nucleotide exchange factor) Cdc24, and the formin Bni1 (named Sep1 in B. cinerea). BcBem1 does not play an important role in regular hyphal growth, but has significant impact on spore formation and germination, on the establishment of conidial anastomosis tubes (CATs) and on virulence. As in other fungi, BcBem1 interacts with the GEF BcCdc24 and the formin BcSep1, indicating that in B. cinerea the apical complex has a similar structure as in yeast. A functional analysis of BcCdc24 suggests that it is essential for growth, since it was not possible to obtain homokaryotic deletion mutants. Heterokaryons of Δcdc24 (supposed to exhibit reduced bccdc24 transcript levels) already show a strong phenotype: an inability to penetrate the host tissue, a significantly reduced growth rate and malformation of conidia, which tend to burst as observed for Δbcbem1. Also the formin BcSep1 has significant impact on hyphal growth and development, whereas the role of the putative ortholog of the yeast scaffold protein Far1 remains open: Δbcfar1 mutants have no obvious phenotypes.
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