ArticlePloS one2014
Nutritional and metabolic requirements for the infection of HeLa cells by Salmonella enterica serovar Typhimurium.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Metabolic strategies of Enterobacteriaceae gut colonization.Nature reviews. Microbiology · 2026Review
- Provision of preferred nutrients to macrophages promotes Salmonella intracellular replication without relying on Type III secretion systems.PLoS pathogens · 2026Article
- Salmonella Typhimurium hijacks a host glucose transporter for intravacuolar proliferation.Cell communication and signaling : CCS · 2026Article
- Review
- The Role ofFoods (Basel, Switzerland) · 2022Article
- Intracellular Salmonella Paratyphi A is motile and differs in the expression of flagella-chemotaxis, SPI-1 and carbon utilization pathways in comparison to intracellular S. Typhimurium.PLoS pathogens · 2022Article
- Dual transcriptome based reconstruction of Salmonella-human integrated metabolic network to screen potential drug targets.PloS one · 2022Article
- Identification of common highly expressed genes of Salmonella Enteritidis by in silico prediction of gene expression and in vitro transcriptomic analysis.Poultry science · 2019Article
- Cyclic-di-GMP regulation promotes survival of a slow-replicating subpopulation of intracellularProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Effective mucosal live attenuated Salmonella vaccine by deleting phosphotransferase system component genes ptsI and crr.Journal of microbiology (Seoul, Korea) · 2019Article
- Review
- Salmonella Co-opts Host Cell Chaperone-mediated Autophagy for Intracellular Growth.The Journal of biological chemistry · 2017Article
- Disruption of glycolytic flux is a signal for inflammasome signaling and pyroptotic cell death.eLife · 2016Article
- Article
- Inherent Variability of Growth Media Impacts the Ability of Salmonella Typhimurium to Interact with Host Cells.PloS one · 2016Article
- Interrelationship between type three secretion system and metabolism in pathogenic bacteria.Frontiers in cellular and infection microbiology · 2014Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Salmonella is the causative agent of a spectrum of human and animal diseases ranging from gastroenteritis to typhoid fever. It is a food--and water--borne pathogen and infects via ingestion followed by invasion of intestinal epithelial cells and phagocytic cells. In this study we employed a mutational approach to define the nutrients and metabolic pathways required by Salmonella enterica serovar Typhimurium during infection of a human epithelial cell line (HeLa). We deleted the key glycolytic genes, pfkA and pfkB to show that S. Typhimurium utilizes glycolysis for replication within HeLa cells; however, glycolysis was not absolutely essential for intracellular replication. Using S. Typhimurium strains deleted for genes encoding components of the phosphotransferase system and glucose transport, we show that glucose is a major substrate required for the intracellular replication of S. Typhimurium in HeLa cells. We also deleted genes encoding enzymes involved in the utilization of gluconeogenic substrates and the glyoxylate shunt and show that neither of these pathways were required for intracellular replication of S. Typhimurium within HeLa cells.
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Registered trials
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