ArticleNature methods2014
CRISPR transcriptional repression devices and layered circuits in mammalian cells.
Article in Nature methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 100 papers.
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Who cites it
100 citing papers in PubMed.
- Designing and testing CRISPRi-based synthetic gene circuits in plants.Nature protocols · 2026Review
- Multi-level precise regulation of gene transcription in the yeast Saccharomyces cerevisiae based on light-sensitive CRISPR/Cas systems.Nucleic acids research · 2026Article
- Dynamic Transcription Machineries in Protocells.Journal of the American Chemical Society · 2025Review
- CRISPRi-based circuits to control gene expression in plants.Nature biotechnology · 2025Article
- Advancing programmable gene expression in plants using CRISPRi-based Boolean gates.Nature biotechnology · 2025Article
- Simulations predict stronger CRISPRi transcriptional repression in plants for identical than heterogeneous gRNA target sites.Synthetic biology (Oxford, England) · 2025Article
- Virtual cells in a virtual microenvironment recapitulate early development-like patterns in human pluripotent stem cell colonies.Stem cell reports · 2023Article
- Application progress of CRISPR/Cas9 genome-editing technology in edible fungi.Frontiers in microbiology · 2023Review
- Functional Synthetic Biology.Synthetic biology (Oxford, England) · 2023Review
- Immunogenicity of CRISPR therapeutics-Critical considerations for clinical translation.Frontiers in bioengineering and biotechnology · 2023Review
- Synthetic gene circuits for cell state detection and protein tuning in human pluripotent stem cells.Molecular systems biology · 2022Article
- gDesigner: computational design of synthetic gRNAs for Cas12a-based transcriptional repression in mammalian cells.NPJ systems biology and applications · 2022Article
- Tutorial: design and execution of CRISPR in vivo screens.Nature protocols · 2022Review
- Intronic gRNAs for the Construction of Minimal Gene Drive Systems.Frontiers in bioengineering and biotechnology · 2022Article
- Application and Prospect of CRISPR/Cas9 Technology in Reversing Drug Resistance of Non-Small Cell Lung Cancer.Frontiers in pharmacology · 2022Review
- RNP-Based Control Systems for Genetic Circuits in Synthetic Biology Beyond CRISPR.Methods in molecular biology (Clifton, N.J.) · 2022Review
- CRISPR-Based Genetic Switches and Other Complex Circuits: Research and Application.Life (Basel, Switzerland) · 2021Review
- Design of time-delayed safety switches for CRISPR gene therapy.Scientific reports · 2021Article
- Article
- Context-aware synthetic biology by controller design: Engineering the mammalian cell.Cell systems · 2021Review
40 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
A key obstacle to creating sophisticated genetic circuits has been the lack of scalable device libraries. Here we present a modular transcriptional repression architecture based on clustered regularly interspaced palindromic repeats (CRISPR) system and examine approaches for regulated expression of guide RNAs in human cells. Subsequently we demonstrate that CRISPR regulatory devices can be layered to create functional cascaded circuits, which provide a valuable toolbox for engineering purposes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.