Evidence map›Paper›PMID 24797271›Full record

ArticlePloS one2014

A toolbox for tuberculosis (TB) diagnosis: an Indian multi-centric study (2006-2008); evaluation of serological assays based on PGL-Tb1 and ESAT-6/CFP10 antigens for TB diagnosis.

Philippe H Lagrange, Satheesh K Thangaraj, Rajeshwar Dayal, Alaka Deshpande, Nirmal K Ganguly, Enrico Girardi, Beenu Joshi, Kiran Katoch, Vishwa M Katoch, Manoj Kumar and 16 more

Open access · goldAbstract readComparative StudyEvaluation StudyMulticenter Study
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 13 citations in OpenAlex.

  1. Diagnostic Potential of the Serological Response to Synthetic Peptides fromInternational journal of peptide research and therapeutics · 2022
    Article
  2. Review
  3. Article
  4. Human Immunology of Tuberculosis.Microbiology spectrum · 2017
    Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

26 authors at 12 institutions in 3 countries.

Philippe H LagrangeService de Microbiologie, Hôpital Saint Louis, Paris, France.
Satheesh K ThangarajBioMérieux, Marcy-l'Etoile, France and New Delhi, India.
Rajeshwar DayalSN Medical College, Agra, India.
Alaka DeshpandeSir J.J. Group of Govt Hosp. & Grant Medical College, Mumbai, India.
Nirmal K GangulyIndian Council of Medical Research, New Delhi, India.
Enrico GirardiDepartment of Epidemiology and Preclinical Research, L. Spallanzani National Institute for Infectious Diseases, Rome, Italy.
Beenu JoshiNational JALMA Institute of Leprosy & Other Mycrobacterial Diseases, Agra, India.
Kiran KatochNational JALMA Institute of Leprosy & Other Mycrobacterial Diseases, Agra, India.
Vishwa M KatochNational JALMA Institute of Leprosy & Other Mycrobacterial Diseases, Agra, India.
Manoj KumarAll India Institute of Medical Sciences, New Delhi, India.
Vemu LakshmiNizam's Institute of Medical Sciences, Hyderabad, India.
Marc LeportierBioMérieux, Marcy-l'Etoile, France and New Delhi, India.
Christophe LonguetFondation Mérieux, Lyon, France.
Subbalaxmi V S MalladiNizam's Institute of Medical Sciences, Hyderabad, India.
Deepali MukerjeeIndian Council of Medical Research, New Delhi, India.
Deepthi NairSafdarjung Hospital, New Delhi, India.
Alamelu RajaNational Institute for Research in Tuberculosis (formerly Tuberculosis Research Center), Chetput, Chennai, India.
Balambal RamanNational Institute for Research in Tuberculosis (formerly Tuberculosis Research Center), Chetput, Chennai, India.
Camilla RodriguesMicrobiology Section, P D Hinduja Hospital & Medical Research Centre, Veer Savarkar Marg Mahim, Mumbai, India.
Pratibha SharmaSafdarjung Hospital, New Delhi, India.
Amit SinghSN Medical College, Agra, India.
Sarman SinghAll India Institute of Medical Sciences, New Delhi, India.
Archana SodhaMicrobiology Section, P D Hinduja Hospital & Medical Research Centre, Veer Savarkar Marg Mahim, Mumbai, India.
Basirudeen Syed Ahamed KabeerNational Institute for Research in Tuberculosis (formerly Tuberculosis Research Center), Chetput, Chennai, India.
Guy VernetFondation Mérieux, Lyon, France.
Delia GolettiDepartment of Epidemiology and Preclinical Research, L. Spallanzani National Institute for Infectious Diseases, Rome, Italy.
National Institute of Research in Tuberculosis · INNational JALMA Institute for Leprosy & Other Mycobacterial Diseases · INAll India Institute of Medical Sciences · INFondation Mérieux · FRIndian Council of Medical Research · INIstituto Nazionale per le Malattie Infettive Lazzaro Spallanzani · ITNizam's Institute of Medical Sciences · INP. D. Hinduja Hospital and Medical Research Centre · INSafdarjang Hospital · INSarojini Naidu Medical College · INGrant Medical College and Sir Jamshedjee Jeejeebhoy Group of Hospitals · INHôpital Saint-Louis · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe aim of this multi-centric prospective study in India was to assess the accuracy of a serological test as an additional tool for diagnosing active tuberculosis (ATB). In particular, an assay based on ELISA using a phenolic glycolipid (PGL-Tb1) or a fusion protein (ESAT-6/CFP10) was compared to the tuberculin skin test (TST) and the microbiological results according to HIV status.

methodsIndividuals with and without ATB and HIV infection were enrolled. Serology and TST results were analyzed per se and in combination with the microbiological data.

resultsAmong the 778 ATB patients, 102 were HIV-infected, 316 HIV-uninfected and 360 had an HIV-unknown status. Of the 945 non-ATB subjects, 559 were at low risk (community adults) and 386 at high risk of M. tuberculosis exposure. Among those with ATB, the sensitivity of ELISA-PGL-Tb1 for ATB was higher than that of ELISA-ESAT-6/CFP10, both in HIV-infected (72.3% versus 63.7%, p = 0.29) and HIV-uninfected/HIV-unknown groups (40.5% versus 28.6%; p<0.0001), whereas the specificity was around 91% for both tests. Sensitivity for ATB increased when the results of the two ELISA were combined, reaching 75.5% in the HIV-infected and 50.9% in the group of HIV-uninfected/HIV-unknown ATB, with a significant decrease of the global specificity (83.9%). Analyzing the ELISA results with the microbiological results, we observed that the sensitivity of both serology tests was independent of the ATB patients' smear microscopy (SM) status and grade. Combining the results of SM with both ELISA, the detection of ATB patients significantly increased (p<0.0001), particularly in those with extrapulmonary TB (up to 45.1%) or HIV infection (up to 83.3%). No significant association was observed between TST and serology results.

conclusionsIn this prospective multi-centric study, the combination of two rapid tests, such as SM and serology, might be useful in detecting ATB, especially in HIV-infected patients.

Indexed as

AdultAntigens, BacterialBacterial LoadEnzyme-Linked Immunosorbent AssayFemaleHIV InfectionsHumansIndiaMaleProspective StudiesROC CurveSensitivity and SpecificitySerologic TestsTuberculin TestTuberculosisAntigens, Bacterial

Identifiers

PMID24797271
PMCPMC4010510
OpenAlexW2068767398

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.