ArticleAnalytical chemistry2014
Urine sample preparation in 96-well filter plates for quantitative clinical proteomics.
Article in Analytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Recent advancements in sample pretreatment methods for glycoproteomics.Biophysics reports · 2026Article
- Atmospheric air plasma pre-activation and customizable covalent functionalization of PVDF-membranes of microtiter filter plates.Scientific reports · 2025Article
- Development of an efficient, effective, and economical technology for proteome analysis.Cell reports methods · 2024Article
- Bottom-Up Proteomics: Advancements in Sample Preparation.International journal of molecular sciences · 2023Review
- Rapid LC-MS assay for targeted metabolite quantification by serial injection into isocratic gradients.Analytical and bioanalytical chemistry · 2023Article
- A multiomics analysis of direct interkingdom dynamics between influenza A virus and Streptococcus pneumoniae uncovers host-independent changes to bacterial virulence fitness.PLoS pathogens · 2022Article
- Label-Free Quantification (LFQ) of Fecal Proteins for Potential Pregnancy Detection in Polar Bears.Life (Basel, Switzerland) · 2022Article
- Oral Microbial Species and Virulence Factors Associated with Oral Squamous Cell Carcinoma.Microbial ecology · 2021Article
- Sample Preparation and Diagnostic Methods for a Variety of Settings: A Comprehensive Review.Molecules (Basel, Switzerland) · 2021Review
- A Universal and High-Throughput Proteomics Sample Preparation Platform.Analytical chemistry · 2021Article
- [Advances in high-throughput proteomic analysis].Se pu = Chinese journal of chromatography · 2021Review
- Protein and Microbial Biomarkers in Sputum Discern Acute and Latent Tuberculosis in Investigation of Pastoral Ethiopian Cohort.Frontiers in cellular and infection microbiology · 2021Article
- Urethral Catheter Biofilms Reveal Plasticity in Bacterial Composition and Metabolism and Withstand Host Immune Defenses in Hypoxic Environment.Frontiers in medicine · 2021Article
- Development of a Microplate Platform for High-Throughput Sample Preparation Based on Microwave Metasurfaces.IEEE access : practical innovations, open solutions · 2021Article
- Lab-on-a-Filter Techniques for Economical, Effective, and Flexible Proteome Analysis.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Differences in plasma proteomes for active tuberculosis, latent tuberculosis and non-tuberculosis mycobacterial lung disease patients with and without ESAT-6/CFP10 stimulation.Proteome science · 2020Article
- Organ-Specific Screening for Protein Damage Using Magnetic Bead Bioreactors and LC-MS/MS.Analytical chemistry · 2020Article
- Unravelling the proteomic landscape of extracellular vesicles in prostate cancer by density-based fractionation of urine.Journal of extracellular vesicles · 2020Article
- Proteomic approaches for characterizing renal cell carcinoma.Clinical proteomics · 2020Review
- Simple Tip-Based Sample Processing Method for Urinary Proteomic Analysis.Analytical chemistry · 2019Article
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6 authors.
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Abstract
Urine is an important, noninvasively collected body fluid source for the diagnosis and prognosis of human diseases. Liquid chromatography mass spectrometry (LC-MS) based shotgun proteomics has evolved as a sensitive and informative technique to discover candidate disease biomarkers from urine specimens. Filter-aided sample preparation (FASP) generates peptide samples from protein mixtures of cell lysate or body fluid origin. Here, we describe a FASP method adapted to 96-well filter plates, named 96FASP. Soluble urine concentrates containing ~10 μg of total protein were processed by 96FASP and LC-MS resulting in 700-900 protein identifications at a 1% false discovery rate (FDR). The experimental repeatability, as assessed by label-free quantification and Pearson correlation analysis for shared proteins among replicates, was high (R ≥ 0.97). Application to urinary pellet lysates which is of particular interest in the context of urinary tract infection analysis was also demonstrated. On average, 1700 proteins (±398) were identified in five experiments. In a pilot study using 96FASP for analysis of eight soluble urine samples, we demonstrated that protein profiles of technical replicates invariably clustered; the protein profiles for distinct urine donors were very different from each other. Robust, highly parallel methods to generate peptide mixtures from urine and other body fluids are critical to increase cost-effectiveness in clinical proteomics projects. This 96FASP method has potential to become a gold standard for high-throughput quantitative clinical proteomics.
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