ArticleFrontiers in plant science2014
An update: improvements in imaging perfluorocarbon-mounted plant leaves with implications for studies of plant pathology, physiology, development and cell biology.
Article in Frontiers in plant science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 66 citations in OpenAlex.
- Rapid reconstitution and kinase-controlled regulation of algal pyrenoid condensates in engineeredbioRxiv : the preprint server for biology · 2026Article
- Best practices in plant fluorescence imaging and reporting: A primer.The Plant cell · 2025Review
- Revealing real-time 3D in vivo pathogen dynamics in plants by label-free optical coherence tomography.Nature communications · 2024Article
- Genetically manipulated chloroplast stromal phosphate levels alter photosynthetic efficiency.Plant physiology · 2024Article
- An optimized live imaging and growth analysis approach for Arabidopsis Sepals.bioRxiv : the preprint server for biology · 2024Article
- An optimized live imaging and multiple cell layer growth analysis approach using Arabidopsis sepals.Frontiers in plant science · 2024Article
- Next-generation ABACUS biosensors reveal cellular ABA dynamics driving root growth at low aerial humidity.Nature plants · 2023Article
- Nonlinear imaging and vibrational spectroscopic analysis of cellulosic fibres treated with COEX® flame-retardant for tapestry preservation.RSC advances · 2022Article
- The development of extracellular vesicle markers for the fungal phytopathogen Colletotrichum higginsianum.Journal of extracellular vesicles · 2022Article
- NuA4 and H2A.Z control environmental responses and autotrophic growth in Arabidopsis.Nature communications · 2022Article
- ClearSeeAlpha: Advanced Optical Clearing for Whole-Plant Imaging.Plant & cell physiology · 2021Article
- Transcriptome Analysis of Potato Infected with the Necrotrophic PathogenPlants (Basel, Switzerland) · 2021Article
- Leaf infiltration in plant science: old method, new possibilities.Plant methods · 2021Review
- Live imaging of microtubule organization, cell expansion, and intercellular space formation in Arabidopsis leaf spongy mesophyll cells.The Plant cell · 2021Article
- Cryo-fluorescence microscopy of high-pressure frozen C. elegans enables correlative FIB-SEM imaging of targeted embryonic stages in the intact worm.Methods in cell biology · 2021Article
- Shape matters: the pitfalls of analyzing mesophyll anatomy.The New phytologist · 2020Article
- Genetic Screens to Target Embryo and Endosperm Pathways in Arabidopsis and Maize.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata.EMBO reports · 2019Article
- An Immune-Responsive Cytoskeletal-Plasma Membrane Feedback Loop in Plants.Current biology : CB · 2018Article
- Tackling Plant Meiosis: From Model Research to Crop Improvement.Frontiers in plant science · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Plant leaves are optically complex, which makes them difficult to image by light microscopy. Careful sample preparation is therefore required to enable researchers to maximize the information gained from advances in fluorescent protein labeling, cell dyes and innovations in microscope technologies and techniques. We have previously shown that mounting leaves in the non-toxic, non-fluorescent perfluorocarbon (PFC), perfluorodecalin (PFD) enhances the optical properties of the leaf with minimal impact on physiology. Here, we assess the use of the PFCs, PFD, and perfluoroperhydrophenanthrene (PP11) for in vivo plant leaf imaging using four advanced modes of microscopy: laser scanning confocal microscopy (LSCM), two-photon fluorescence microscopy, second harmonic generation microscopy, and stimulated Raman scattering (SRS) microscopy. For every mode of imaging tested, we observed an improved signal when leaves were mounted in PFD or in PP11, compared to mounting the samples in water. Using an image analysis technique based on autocorrelation to quantitatively assess LSCM image deterioration with depth, we show that PP11 outperformed PFD as a mounting medium by enabling the acquisition of clearer images deeper into the tissue. In addition, we show that SRS microscopy can be used to image PFCs directly in the mesophyll and thereby easily delimit the "negative space" within a leaf, which may have important implications for studies of leaf development. Direct comparison of on and off resonance SRS micrographs show that PFCs do not to form intracellular aggregates in live plants. We conclude that the application of PFCs as mounting media substantially increases advanced microscopy image quality of living mesophyll and leaf vascular bundle cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.