ArticleFrontiers in microbiology2014
Biofilm formation of Clostridium perfringens and its exposure to low-dose antimicrobials.
Article in Frontiers in microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 73 citations in OpenAlex.
- Innovative Approaches Against Planktonic and Biofilm-ProducingVeterinary sciences · 2026Review
- Biofilm Formation and Spore-Mediated Persistence of Clostridium perfringens in Meat and Poultry Processing Environments and Their Implications for Control Strategies.Journal of food science · 2026Review
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- Intestinal overgrowth ofFrontiers in veterinary science · 2026Article
- In Vitro Insights into Bacteriocin-Mediated Modulation of Chicken Cecal Microbiota.International journal of molecular sciences · 2025Article
- Comparison of Taxonomic Resolutions of Various Typing Methods forIndian journal of microbiology · 2024Article
- Study of the Antibacterial Activity of Rich Polyphenolic Extracts Obtained fromAntibiotics (Basel, Switzerland) · 2023Article
- The Role of Microbiota in Pancreatic Cancer.Cancers · 2023Review
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- Characterization and Genomic Analysis of a Novel Lytic Phage DCp1 againstInternational journal of molecular sciences · 2023Article
- A Broad-Spectrum Phage Endolysin (LysCP28) Able to Remove Biofilms and InactivateFoods (Basel, Switzerland) · 2023Article
- Genetic Relatedness, Antibiotic Resistance, and Effect of Silver Nanoparticle on Biofilm Formation byVeterinary sciences · 2022Article
- Intratumor Microbiome Analysis Identifies Positive Association BetweenFrontiers in immunology · 2022Article
- Epigallocatechin gallate and Lactobacillus plantarum culture supernatants exert bactericidal activity and reduce biofilm formation in Clostridium perfringens.Folia microbiologica · 2021Article
- Bacteriocin-Like Inhibitory Substance (BLIS) Activity of Enterococcus faecium DB1 Against Biofilm Formation by Clostridium perfringens.Probiotics and antimicrobial proteins · 2021Article
- Biofilm and Spore Formation ofAntibiotics (Basel, Switzerland) · 2021Article
- Temperature-regulated heterogeneous extracellular matrix gene expression defines biofilm morphology in Clostridium perfringens.NPJ biofilms and microbiomes · 2020Article
- Antimicrobial Resistance inMicrobiology spectrum · 2020Review
- Green Alternatives to Synthetic Antioxidants, Antimicrobials, Nitrates, and Nitrites in Clean Label Spanish Chorizo.Antioxidants (Basel, Switzerland) · 2019Article
- Liberibacter crescens biofilm formation in vitro: establishment of a model system for pathogenic 'Candidatus Liberibacter spp.'Scientific reports · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clostridium perfringens is an opportunistic pathogen that can cause food poisoning in humans and various enterotoxemia in animal species. Very little is known on the biofilm of C. perfringens and its exposure to subminimal inhibitory concentrations of antimicrobials. This study was undertaken to address these issues. Most of the C. perfringens human and animal isolates tested in this study were able to form biofilm (230/277). Porcine clinical isolates formed significantly more biofilm than the porcine commensal isolates. A subgroup of clinical and commensal C. perfringens isolates was randomly selected for further characterization. Biofilm was found to protect C. perfringens bacterial cells from exposure to high concentrations of tested antimicrobials. Exposure to low doses of some of these antimicrobials tended to lead to a diminution of the biofilm formed. However, a few isolates showed an increase in biofilm formation when exposed to low doses of tylosin, bacitracin, virginiamycin, and monensin. Six isolates were randomly selected for biofilm analysis using scanning laser confocal microscopy. Of those, four produced more biofilm in presence of low doses of bacitracin whereas biofilms formed without bacitracin were thinner and less elevated. An increase in the area occupied by bacteria in the biofilm following exposure to low doses of bacitracin was also observed in the majority of isolates. Morphology examination revealed flat biofilms with the exception of one isolate that demonstrated a mushroom-like biofilm. Matrix composition analysis showed the presence of proteins, beta-1,4 linked polysaccharides and extracellular DNA, but no poly-beta-1,6-N-acetyl-D-glucosamine. This study brings new information on the biofilm produced by C. perfringens and its exposure to low doses of antimicrobials.
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