ArticleThe EMBO journal1989
Anion transport in oocytes of Xenopus laevis induced by expression of mouse erythroid band 3 protein--encoding cRNA and of a cRNA derivative obtained by site-directed mutagenesis at the stilbene disulfonate binding site.
Article in The EMBO journal, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 65 citations in OpenAlex.
- Structure and Function of SLC4 Family [Formula: see text] Transporters.Frontiers in physiology · 2015Article
- The divergence, actions, roles, and relatives of sodium-coupled bicarbonate transporters.Physiological reviews · 2013Review
- Using fluorometry and ion-sensitive microelectrodes to study the functional expression of heterologously-expressed ion channels and transporters in Xenopus oocytes.Methods (San Diego, Calif.) · 2010Review
- A conductive pathway generated from fragments of the human red cell anion exchanger AE1.The Journal of physiology · 2007Article
- The SLC4 family of HCO 3 - transporters.Pflugers Archiv : European journal of physiology · 2004Review
- Multiple transport functions of a red blood cell anion exchanger, tAE1: its role in cell volume regulation.The Journal of physiology · 2001Article
- Familial distal renal tubular acidosis is associated with mutations in the red cell anion exchanger (Band 3, AE1) gene.The Journal of clinical investigation · 1997Article
- Electrogenic sulfate/chloride exchange in Xenopus oocytes mediated by murine AE1 E699Q.The Journal of general physiology · 1997Article
- Functional cell surface expression of the anion transport domain of human red cell band 3 (AE1) in the yeast Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- The effects of glycophorin A on the expression of the human red cell anion transporter (band 3) in Xenopus oocytes.The Journal of membrane biology · 1994Article
- Endogenous Na(+)-K+ (or NH4+)-2Cl- cotransport in Rana oocytes; anomalous effect of external NH4+ on pHi.The Journal of physiology · 1994Article
- Amino acid sequence of band-3 protein from rainbow trout erythrocytes derived from cDNA.The Biochemical journal · 1992Article
- Exploration of the functional significance of the stilbene disulfonate binding site in mouse band 3 by site-directed mutagenesis.Biophysical journal · 1992Article
- Role of Lys 558 and Lys 869 in substrate and inhibitor binding to the murine band 3 protein: a study of the effects of site-directed mutagenesis of the band 3 protein expressed in the oocytes of Xenopus laevis.The Journal of membrane biology · 1992Article
- Ectopic expression of the erythrocyte band 3 anion exchange protein, using a new avian retrovirus vector.Journal of virology · 1990Article
- The chloroplast import receptor is an integral membrane protein of chloroplast envelope contact sites.The Journal of cell biology · 1990Article
- Protein engineering and site-directed mutagenesis. Patents and literature.Applied biochemistry and biotechnology · 1990Article
- Use of Xenopus oocytes for the functional expression of plasma membrane proteins.The Journal of membrane biology · 1990Review
- Functional expression and subcellular localization of an anion exchanger cloned from choroid plexus.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
- The SLC4 family of bicarbonate (HCO₃⁻) transporters.Molecular aspects of medicineReview
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A vector was constructed containing a cDNA for mouse band 3 obtained from Demuth et al. (1986, EMBO J., 5, 1205-1214), a synthetic linker (containing 5'-non-translated region, start codon and a coding region for the first 12 N-terminal amino acids), and RNA polymerase promoters suitable for in vitro transcription of cRNA. After injection of the cRNA into the cytoplasm of Xenopus oocytes and incubation for 16 h, expression of mouse band 3 was demonstrated by immunoprecipitation, immunohistochemical methods and influx or efflux measurements with 36Cl-. Antisense cRNA inhibits the expression. Lysines 558 and 561 were replaced by asparagines using oligonucleotide-directed mutagenesis. Like the original band 3, the mutant shows stilbene disulfonate-inhibitable anion exchange. However, in contrast to the original band 3, inhibition by 4,4'-diisothiocyano dihydrostilbene-2,2'-disulfonate (H2DIDS) is no longer irreversible. This indicates that thiourea bond formation between H2DIDS and band 3 involves one of the two modified lysine residues. It also shows that the two lysine residues are not essential for the execution of the anion transport function of band 3. The results described suggest that the cDNA clone of Demuth et al. (1986) encodes a protein with properties that are representative for the properties of the bulk of the band 3 protein in the plasma membrane of the red cell of the mouse.
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