ArticleApplied and environmental microbiology2014
Beyond the matrix-assisted laser desorption ionization (MALDI) biotyping workflow: in search of microorganism-specific tryptic peptides enabling discrimination of subspecies.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed.
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- Taxonomic Diversity and Antimicrobial Potential of Thermophilic Bacteria from Two Extreme Algerian Hot Springs.Microorganisms · 2025Article
- Antagonistic Effects of Lactic Acid Bacteria Isolated from Ethiopian Traditional Fermented Foods and Beverages Against Foodborne Pathogens.Probiotics and antimicrobial proteins · 2025Article
- Unde venis? Bacterial resistance from environmental reservoirs to lettuce: tracking microbiome and resistome over a growth period.FEMS microbiology ecology · 2024Article
- Spatiotemporal multi-omics: exploring molecular landscapes in aging and regenerative medicine.Military Medical Research · 2024Review
- Private and well drinking water are reservoirs for antimicrobial resistant bacteria.npj antimicrobials and resistance · 2024Article
- MALDI-TOF as a powerful tool for identifying and differentiating closely related microorganisms: the strange case of three reference strains of Paenibacillus polymyxa.Scientific reports · 2024Article
- Lipidomics Characterization of the Microbiome in People with Diabetic Foot Infection Using MALDI-TOF MS.Analytical chemistry · 2023Article
- A Novel Robust Screening Assay IdentifiesMicroorganisms · 2023Article
- PepGM: a probabilistic graphical model for taxonomic inference of viral proteome samples with associated confidence scores.Bioinformatics (Oxford, England) · 2023Article
- Protein biomarker elucidation for the verification of biological agents in the taxonomic group of Gammaproteobacteria using tandem mass spectrometry.World journal of microbiology & biotechnology · 2021Article
- Characterization ofMicroorganisms · 2020Article
- Transferable Extended-Spectrum β-Lactamase (ESBL) Plasmids inMicroorganisms · 2020Article
- Mass Spectrometry: A Rosetta Stone to Learn How Fungi Interact and Talk.Life (Basel, Switzerland) · 2020Review
- Assessing the intestinal carriage rates of vancomycin-resistant enterococci (VRE) at a tertiary care hospital in Hungary.Folia microbiologica · 2020Article
- Biodegradation of recalcitrant compounds and phthalates by culturable bacteria isolated from Liometopum apiculatum microbiota.World journal of microbiology & biotechnology · 2020Article
- Elucidation of protein biomarkers for verification of selected biological warfare agents using tandem mass spectrometry.Scientific reports · 2020Article
- Fast and Accurate Bacterial Species Identification in Urine Specimens Using LC-MS/MS Mass Spectrometry and Machine Learning.Molecular & cellular proteomics : MCP · 2019Article
- Pathogen proteotyping: A rapidly developing application of mass spectrometry to address clinical concerns.Clinical mass spectrometry (Del Mar, Calif.) · 2019Review
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5 authors.
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Abstract
A well-accepted method for identification of microorganisms uses matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) coupled to analysis software which identifies and classifies the organism according to its ribosomal protein spectral profile. The method, called MALDI biotyping, is widely used in clinical diagnostics and has partly replaced conventional microbiological techniques such as biochemical identification due to its shorter time to result (minutes for MALDI biotyping versus hours or days for classical phenotypic or genotypic identification). Besides its utility for identifying bacteria, MS-based identification has been shown to be applicable also to yeasts and molds. A limitation to this method, however, is that accurate identification is most reliably achieved on the species level on the basis of reference mass spectra, making further phylogenetic classification unreliable. Here, it is shown that combining tryptic digestion of the acid/organic solvent extracted (classical biotyping preparation) and resolubilized proteins, nano-liquid chromatography (nano-LC), and subsequent identification of the peptides by MALDI-tandem TOF (MALDI-TOF/TOF) mass spectrometry increases the discrimination power to the level of subspecies. As a proof of concept, using this targeted proteomics workflow, we have identified subspecies-specific biomarker peptides for three Salmonella subspecies, resulting in an extension of the mass range and type of proteins investigated compared to classical MALDI biotyping. This method therefore offers rapid and cost-effective identification and classification of microorganisms at a deeper taxonomic level.
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