ArticleApplied and environmental microbiology2014
Metatranscriptome of an anaerobic benzene-degrading, nitrate-reducing enrichment culture reveals involvement of carboxylation in benzene ring activation.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 116 citations in OpenAlex.
- Putative Mechanisms for Initial Reaction of Anaerobic Benzene Degradation Presumably Involving a Flavoprotein.Environmental microbiology · 2026Article
- Identification of a highly expressed gene cluster likely coding for benzene activation enzymes in a methanogenic enrichment culture.Applied and environmental microbiology · 2026Article
- Unlocking microbial potential: advances in omics and bioinformatics for aromatic hydrocarbon degradation.World journal of microbiology & biotechnology · 2025Review
- Isolation, Molecular, and Metabolic Profiling of Benzene-Remediating Bacteria Inhabiting the Tannery Industry Soil.Polish journal of microbiology · 2025Article
- Disentangling the microbial genomic traits associated with aromatic hydrocarbon degradation in a jet fuel-contaminated aquifer.Biodegradation · 2024Article
- Metagenomic and genomic sequences from a nitrate-reducing benzene-degrading enrichment culture.Microbiology resource announcements · 2024Article
- Draft genome of an anaerobic nitrate-reducing, benzene-degrading member of the orderMicrobiology resource announcements · 2024Article
- Naphthalene Carboxylation in the Sulfate-Reducing Enrichment Culture N47 Is Proposed to Proceed via 1,3-Dipolar Cycloaddition to the Cofactor Prenylated Flavin Mononucleotide.Applied and environmental microbiology · 2023Article
- Genetic diversity into a novel free-living species ofFrontiers in microbiology · 2023Article
- Potential for the anaerobic oxidation of benzene and naphthalene in thermophilic microorganisms from the Guaymas Basin.Frontiers in microbiology · 2023Article
- Transient Oxygen Exposure Causes Profound and Lasting Changes to a Benzene-Degrading Methanogenic Community.Environmental science & technology · 2022Article
- Decoding the roles of extremophilic microbes in the anaerobic environments: Past, Present, and Future.Current research in microbial sciences · 2022Review
- High biodiversity in a benzene-degrading nitrate-reducing culture is sustained by a few primary consumers.Communications biology · 2021Article
- Structure and Mechanism ofACS catalysis · 2021Article
- Anaerobic benzene mineralization by natural microbial communities from Niger Delta.Biodegradation · 2021Article
- Optimized Cultivation and Syntrophic Relationship of Anaerobic Benzene-Degrading Enrichment Cultures under Methanogenic Conditions.Microbes and environments · 2021Article
- Vertical Stratification of Dissolved Organic Matter Linked to Distinct Microbial Communities in Subtropic Estuarine Sediments.Frontiers in microbiology · 2021Article
- Enzymatic C-H activation of aromatic compounds through CONature chemical biology · 2020Article
- Combined Use of Diagnostic Fumarate Addition Metabolites and Genes Provides Evidence for Anaerobic Hydrocarbon Biodegradation in Contaminated Groundwater.Microorganisms · 2020Article
- Omics Approaches to Pesticide Biodegradation.Current microbiology · 2020Review
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The enzymes involved in the initial steps of anaerobic benzene catabolism are not known. To try to elucidate this critical step, a metatranscriptomic analysis was conducted to compare the genes transcribed during the metabolism of benzene and benzoate by an anaerobic benzene-degrading, nitrate-reducing enrichment culture. RNA was extracted from the mixed culture and sequenced without prior mRNA enrichment, allowing simultaneous examination of the active community composition and the differential gene expression between the two treatments. Ribosomal and mRNA sequences attributed to a member of the family Peptococcaceae from the order Clostridiales were essentially only detected in the benzene-amended culture samples, implicating this group in the initial catabolism of benzene. Genes similar to each of two subunits of a proposed benzene-carboxylating enzyme were transcribed when the culture was amended with benzene. Anaerobic benzoate degradation genes from strict anaerobes were transcribed only when the culture was amended with benzene. Genes for other benzoate catabolic enzymes and for nitrate respiration were transcribed in both samples, with those attributed to an Azoarcus species being most abundant. These findings indicate that the mineralization of benzene starts with its activation by a strict anaerobe belonging to the Peptococcaceae, involving a carboxylation step to form benzoate. These data confirm the previously hypothesized syntrophic association between a benzene-degrading Peptococcaceae strain and a benzoate-degrading denitrifying Azoarcus strain for the complete catabolism of benzene with nitrate as the terminal electron acceptor.
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