ArticleBiology open2014
H3K79 methylation: a new conserved mark that accompanies H4 hyperacetylation prior to histone-to-protamine transition in Drosophila and rat.
Article in Biology open, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Chromatin environment-dependent effects of DOT1L on gene expression in male germ cells.Communications biology · 2025Article
- miRNA/siRNA-directed pathway to produce noncoding piRNAs from endogenous protein-coding regions ensuresScience advances · 2023Article
- DOT1L regulates chromatin reorganization and gene expression during sperm differentiation.EMBO reports · 2023Article
- DOT1L promotes spermatid differentiation by regulating expression of genes required for histone-to-protamine replacement.Development (Cambridge, England) · 2023Article
- Nucleosomes in mammalian sperm: conveying paternal epigenetic inheritance or subject to reprogramming between generations?Current opinion in genetics & development · 2023Review
- Connecting the DOTs on Cell Identity.Frontiers in cell and developmental biology · 2022Review
- A novel posttranslational modification of histone, H3 S-sulfhydration, is down-regulated in asthenozoospermic sperm.Journal of assisted reproduction and genetics · 2021Article
- Genome-wide occupancy reveals the localization of H1T2 (H1fnt) to repeat regions and a subset of transcriptionally active chromatin domains in rat spermatids.Epigenetics & chromatin · 2021Article
- Maternal DOT1L is dispensable for mouse development.Scientific reports · 2020Article
- The Interplay Between Replacement and Retention of Histones in the Sperm Genome.Frontiers in genetics · 2020Review
- Article
- Nejire/dCBP-mediated histone H3 acetylation during spermatogenesis is essential for male fertility in Drosophila melanogaster.PloS one · 2018Article
- A Decade of Exploring the Mammalian Sperm Epigenome: Paternal Epigenetic and Transgenerational Inheritance.Frontiers in cell and developmental biology · 2018Review
- SLY regulates genes involved in chromatin remodeling and interacts with TBL1XR1 during sperm differentiation.Cell death and differentiation · 2017Article
- Epigenetic regulation of the histone-to-protamine transition during spermiogenesis.Reproduction (Cambridge, England) · 2016Review
- Epigenetic Remodeling in Male Germline Development.Stem cells international · 2016Review
- Epigenetic regulation of germ cells-remember or forget?Current opinion in genetics & development · 2015Review
- Multimerization of Drosophila sperm protein Mst77F causes a unique condensed chromatin structure.Nucleic acids research · 2015Article
- The histone methyltransferase DOT1L: regulatory functions and a cancer therapy target.American journal of cancer research · 2015Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During spermiogenesis, haploid spermatids undergo extensive chromatin remodeling events in which histones are successively replaced by more basic protamines to generate highly compacted chromatin. Here we show for the first time that H3K79 methylation is a conserved feature preceding the histone-to-protamine transition in Drosophila melanogaster and rat. During Drosophila spermatogenesis, the Dot1-like methyltransferase Grappa (Gpp) is primarily expressed in canoe stage nuclei. The corresponding H3K79 methylation is a histone modification that precedes the histone-to-protamine transition and correlates with histone H4 hyperacetylation. When acetylation was inhibited in cultured Drosophila testes, nuclei were smaller and chromatin was compact, Gpp was little synthesized, H3K79 methylation was strongly reduced, and protamines were not synthesized. The Gpp isoform Gpp-D has a unique C-terminus, and Gpp is essential for full fertility. In rat, H3K79 methylation also correlates with H4 hyperacetylation but not with active RNA polymerase II, which might point towards a conserved function in chromatin remodeling during the histone-to-protamine transition in both Drosophila and rat.
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