ArticleDiabetologia2014
Insulin secretion from beta cells in intact mouse islets is targeted towards the vasculature.
Article in Diabetologia, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 93 citations in OpenAlex.
- Heterogeneous expression patterns of the T2D-associated kinesin-4 KIF21A in pancreatic islet endocrine cells.Molecular metabolism · 2026Article
- Applications of Nanopipettes in Scanning Ion Conductance Microscopy for High-Spatial-Resolution Topographic Imaging and Sensing in Single Cells.ACS measurement science au · 2026Review
- Submembrane liprin-α1 clusters spatially localize insulin granule fusion.The Journal of cell biology · 2025Article
- Engineered vasculature induces functional maturation of pluripotent stem cell-derived islet organoids.Developmental cell · 2025Article
- Assessing acute pancreatitis: A novel method combining live cell imaging with tissue damage evaluation.Histology and histopathology · 2025Article
- Directed insulin secretion from beta cells occurs at cortical sites devoid of microtubules at the edges of ELKS/LL5β patches.Molecular biology of the cell · 2025Article
- A Comprehensive Workflow for Imaging Live Insulin Secretion Events and Granules in Intact Islets.bioRxiv : the preprint server for biology · 2025Article
- Directed insulin secretion from beta cells occurs at cortical sites devoid of microtubules at the edges of ELKS/LL5β patches.bioRxiv : the preprint server for biology · 2025Article
- Biphasic glucose-stimulated insulin secretion over decades: a journey from measurements and modeling to mechanistic insights.Life metabolism · 2025Review
- Exploring pancreatic beta-cell subgroups and their connectivity.Nature metabolism · 2024Review
- Capillary contact points determine beta cell polarity, control secretion and are disrupted in the db/db mouse model of diabetes.Diabetologia · 2024Article
- Readily releasable β cells with tight CaNature metabolism · 2024Article
- Bridging the Gap: Pancreas Tissue Slices From Organ and Tissue Donors for the Study of Diabetes Pathogenesis.Diabetes · 2024Review
- Unlocking the Potential of Collagenases: Structures, Functions, and Emerging Therapeutic Horizons.Biodesign research · 2024Article
- Advanced Imaging Techniques for the Characterization of Subcellular Organelle Structure in Pancreatic Islet β Cells.Comprehensive Physiology · 2023Article
- Preparation of Whole-mount Mouse Islets on Vascular Extracellular Matrix for Live Islet Cell Microscopy.Bio-protocol · 2023Article
- Rediscovering Primary Cilia in Pancreatic Islets.Diabetes & metabolism journal · 2023Review
- Insulin secretion hot spots in pancreatic β cells as secreting adhesions.Frontiers in cell and developmental biology · 2023Article
- Glucose-Dependent Insulin Secretion from β Cell Spheroids Is Enhanced by Embedding into Softer Alginate Hydrogels Functionalised with RGD Peptide.Bioengineering (Basel, Switzerland) · 2022Article
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Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aims/hypothesisWe set out to test the hypothesis that insulin secretion from beta cells is targeted towards the vasculature.
methodsThe spatial location of granule fusion was identified by live-cell two-photon imaging of mouse pancreatic beta cells within intact islets, using sulforhodamine B labelling. Three-dimensional (3D) immunofluorescence of pancreatic slices was used to identify the location of proteins associated with neuronal synapses.
resultsWe demonstrated an asymmetric, non-random, distribution of sites of insulin granule fusion in response to glucose and focal targeting of insulin granule secretion to the beta cell membrane facing the vasculature. 3D immunofluorescence of islets showed that structural proteins, such as liprin, piccolo and Rab2-interacting molecule, normally associated with neuronal presynaptic targeting, were present in beta cells and enriched at the vascular face. In contrast, we found that syntaxin 1A and synaptosomal-associated protein 25 kDa (SNAP25) were relatively evenly distributed across the beta cells. CONCLUSIONS/
interpretationOur results show that beta cells in situ, within intact islets, are polarised and target insulin secretion. This evidence for an 'endocrine synapse' has wide implications for our understanding of stimulus-secretion coupling in healthy islets and in disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.