ArticleJournal of bacteriology2014
Expression of each cistron in the gal operon can be regulated by transcription termination and generation of a galk-specific mRNA, mK2.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 26 citations in OpenAlex.
- Disruption of Spore Coat Integrity inCurrent issues in molecular biology · 2025Article
- Rho-dependent termination and RNase E-mediated cleavage: dual pathways for RNA 3' end processing in polycistronic mRNA.Journal of bacteriology · 2025Article
- Transcription Needs Translation Initiation of the Downstream Gene to Continue Downstream at Intercistronic Junctions in E. Coli.Current microbiology · 2024Article
- Article
- Identification of a Rho-Dependent Termination SiteMicrobiology spectrum · 2023Article
- Systematic Comparison and Rational Design of Theophylline Riboswitches for Effective Gene Repression.Microbiology spectrum · 2023Article
- sRNA expedites polycistronic mRNA decay inFrontiers in molecular biosciences · 2023Article
- Article
- Dual-function AzuCR RNA modulates carbon metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- AnBio-protocol · 2022Article
- sRNA-mediated regulation of gal mRNA in E. coli: Involvement of transcript cleavage by RNase E together with Rho-dependent transcription termination.PLoS genetics · 2021Article
- Translation Initiation Control of RNase E-Mediated Decay of PolycistronicFrontiers in molecular biosciences · 2020Article
- Processing generates 3' ends of RNA masking transcription termination events in prokaryotes.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Visualization of RNA 3' ends inBio-protocol · 2018Article
- Two-level inhibition of galK expression by Spot 42: Degradation of mRNA mK2 and enhanced transcription termination before the galK gene.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gal operon of Escherichia coli has 4 cistrons, galE, galT, galK, and galM. In our previous report (H. J. Lee, H. J. Jeon, S. C. Ji, S. H. Yun, H. M. Lim, J. Mol. Biol. 378: 318-327, 2008), we identified 6 different mRNA species, mE1, mE2, mT1, mK1, mK2, and mM1, in the gal operon and mapped these mRNAs. The mRNA map suggests a gradient of gene expression known as natural polarity. In this study, we investigated how the mRNAs are generated to understand the cause of natural polarity. Results indicated that mE1, mT1, mK1, and mM1, whose 3' ends are located at the end of each cistron, are generated by transcription termination. Since each transcription termination is operating with a certain frequency and those 4 mRNAs have 5' ends at the transcription initiation site(s), these transcription terminations are the basic cause of natural polarity. Transcription terminations at galE-galT and galT-galK junctions, making mE1 and mT1, are Rho dependent. However, the terminations to make mK1 and mM1 are partially Rho dependent. The 5' ends of mK2 are generated by an endonucleolytic cleavage of a pre-mK2 by RNase P, and the 3' ends are generated by Rho termination 260 nucleotides before the end of the operon. The 5' portion of pre-mK2 is likely to become mE2. These results also suggested that galK expression could be regulated through mK2 production independent from natural polarity.
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