ArticleJournal of bacteriology2014
Novel MntR-independent mechanism of manganese homeostasis in Escherichia coli by the ribosome-associated protein HflX.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 30 citations in OpenAlex.
- Proteomic profiling of zinc homeostasis mechanisms in Pseudomonas aeruginosa through data-dependent and data-independent acquisition mass spectrometry.Metallomics : integrated biometal science · 2025Article
- Structurally distinct manganese-sensing riboswitch aptamers regulate different expression platform architectures.Nucleic acids research · 2025Article
- Proteomic profiling of zinc homeostasis mechanisms inbioRxiv : the preprint server for biology · 2025Article
- A riboswitch-controlled TerC family transporter Alx tunes intracellular manganese concentration inJournal of bacteriology · 2024Article
- Insights into the function of the chloroplastic ribosome-associated GTPase high frequency of lysogenization X inPlant direct · 2024Article
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- Manganese transporters regulate the resumption of replication in hydrogen peroxide-stressed Escherichia coli.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2023Article
- Development of bacterial resistance induced by low concentration of two-dimensional black phosphorusRSC advances · 2022Article
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- Trends and potential human health risk of trace elements accumulated in transplanted blue mussels during restoration activities of Flekkefjord fjord (Southern Norway).Environmental monitoring and assessment · 2022Article
- Genetic Redundancy in Iron and Manganese Transport in the Metabolically Versatile Bacterium Rhodopseudomonas palustris TIE-1.Applied and environmental microbiology · 2020Article
- Manganese dioxide coating reduces bacterial adhesion and infection in silicon implants in animal model.World journal of urology · 2020Article
- Impact of Different Trace Elements on the Growth and Proteome of Two Strains ofFrontiers in microbiology · 2020Article
- Molecular and Biochemical Characterization of YeeF/YezG, a Polymorphic Toxin-Immunity Protein Pair FromFrontiers in microbiology · 2020Article
- The Impact of the Stringent Response on TRAFAC GTPases and Prokaryotic Ribosome Assembly.Cells · 2019Review
- Structural modules of the stress-induced protein HflX: an outlook on its evolution and biological role.Current genetics · 2019Review
- HflX protein protectsJournal of biosciences · 2018Article
- Structural, functional and biological insights into the role of Mycobacterium tuberculosis VapBC11 toxin-antitoxin system: targeting a tRNase to tackle mycobacterial adaptation.Nucleic acids research · 2018Article
- Affected energy metabolism under manganese stress governs cellular toxicity.Scientific reports · 2017Article
- Characterization of the autophosphorylation property of HflX, a ribosome-binding GTPase from Escherichia coli.FEBS open bio · 2016Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Manganese is a micronutrient required for activities of several important enzymes under conditions of oxidative stress and iron starvation. In Escherichia coli, the manganese homeostasis network primarily constitutes a manganese importer (MntH) and an exporter (MntP), which are regulated by the MntR dual regulator. In this study, we find that deletion of E. coli hflX, which encodes a ribosome-associated GTPase with unknown function, renders extreme manganese sensitivity characterized by arrested cell growth, filamentation, lower rate of replication, and DNA damage. We demonstrate that perturbation by manganese induces unprecedented influx of manganese in ΔhflX cells compared to that in the wild-type E. coli strain. Interestingly, our study indicates that the imbalance in manganese homeostasis in the ΔhflX strain is independent of the MntR regulon. Moreover, the influx of manganese leads to a simultaneous influx of zinc and inhibition of iron import in ΔhflX cells. In order to review a possible link of HflX with the λ phage life cycle, we performed a lysis-lysogeny assay to show that the Mn-perturbed ΔhflX strain reduces the frequency of lysogenization of the phage. This observation raises the possibility that the induced zinc influx in the manganese-perturbed ΔhflX strain stimulates the activity of the zinc-metalloprotease HflB, the key determinant of the lysis-lysogeny switch. Finally, we propose that manganese-mediated autophosphorylation of HflX plays a central role in manganese, zinc, and iron homeostasis in E. coli cells.
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