Evidence map›Paper›PMID 24794563›Full record

ArticleJournal of bacteriology2014

NtrBC and Nac contribute to efficient Shigella flexneri intracellular replication.

Chelsea D Waddell, Thomas J Walter, Sophia A Pacheco, Georgiana E Purdy, Laura J Runyen-Janecky

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Catabolism of serine enantiomers represses enterohemorrhagicProceedings of the National Academy of Sciences of the United States of America · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Chelsea D WaddellDepartment of Molecular Microbiology and Immunology, Oregon Health and Sciences University, Portland, Oregon, USA.
Thomas J WalterDepartment of Biology, University of Richmond, Richmond, Virginia, USA.
Sophia A PachecoDepartment of Molecular Microbiology and Immunology, Oregon Health and Sciences University, Portland, Oregon, USA.
Georgiana E PurdyDepartment of Molecular Microbiology and Immunology, Oregon Health and Sciences University, Portland, Oregon, USA purdyg@ohsu.edu lrunyenj@richmond.edu.
Laura J Runyen-JaneckyDepartment of Biology, University of Richmond, Richmond, Virginia, USA purdyg@ohsu.edu lrunyenj@richmond.edu.

Funding

The Evolution of Protein FlexibilityP20RR016448 · NCRR · UNIVERSITY OF IDAHO · PI BROWN, CELESTE J · 2002 to 2011
$19.3M
Center for Research on Processes in EvolutionP20GM103397 · NIGMS · UNIVERSITY OF IDAHO · PI FORNEY, LARRY J · 2012 to 2012
$1.9M
Role of Shigella two component regulation systems in intracellular adaptationR15AI075330 · NIAID · UNIVERSITY OF RICHMOND · PI RUNYEN-JANECKY, LAURA JANE · 2007 to 2007
$193k
NCRR NIH HHS P20 RR016448NCRR NIH HHS P20RR016448-10NIAID NIH HHS AI075330NIAID NIH HHS R15 AI075330NIGMS NIH HHS P20 GM103397NIGMS NIH HHS P20GM103397-10
6 · The paper itself

Abstract

Shigella flexneri two-component regulatory systems (TCRS) are responsible for sensing changes in environmental conditions and regulating gene expression accordingly. We examined 12 TCRS that were previously uncharacterized for potential roles in S. flexneri growth within the eukaryotic intracellular environment. We demonstrate that the TCRS EvgSA, NtrBC, and RstBA systems are required for wild-type plaque formation in cultured epithelial cells. The phenotype of the NtrBC mutant depended in part on the Nac transcriptional regulator. Microarray analysis was performed to identify S. flexneri genes differentially regulated by the NtrBC system or Nac in the intracellular environment. This study contributes to our understanding of the transcriptional regulation necessary for Shigella to effectively adapt to the mammalian host cell.

Indexed as

Bacterial ProteinsCell LineGene Expression Regulation, BacterialHumansIntestinesProtein Array AnalysisRegulatory Elements, TranscriptionalRNA, BacterialShigella flexneriBacterial ProteinsRNA, Bacterial

Identifiers

PMID24794563
PMCPMC4097594

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.