ArticleJournal of bacteriology2014
A putative bifunctional histidine kinase/phosphatase of the HWE family exerts positive and negative control on the Sinorhizobium meliloti general stress response.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- An HWE-Family Histidine Kinase Modulates Brucella Cell Envelope Properties and Host Innate Immune Response.Molecular microbiology · 2025Article
- The sensor histidine kinase PhcS participates in the regulation of quorum sensing-dependent virulence genes inMicrobiology spectrum · 2025Article
- Insights into the structure and function of the histidine kinase ComP from Bacillus amyloliquefaciens based on molecular modeling.Bioscience reports · 2022Article
- Extracytoplasmic Function σ Factors as Tools for Coordinating Stress Responses.International journal of molecular sciences · 2021Review
- Complex general stress response regulation in Sphingomonas melonis Fr1 revealed by transcriptional analyses.Scientific reports · 2019Article
- Stress-inducible NHEJ in bacteria: function in DNA repair and acquisition of heterologous DNA.Nucleic acids research · 2019Article
- MostmSphere · 2018Article
- Article
- Phosphorelay through the bifunctional phosphotransferase PhyT controls the general stress response in an alphaproteobacterium.PLoS genetics · 2018Article
- Structure and function of HWE/HisKA2-family sensor histidine kinases.Current opinion in microbiology · 2017Review
- A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium Sinorhizobium meliloti.Scientific reports · 2016Article
- Systematic Mutational Analysis of Histidine Kinase Genes in the Nosocomial Pathogen Stenotrophomonas maltophilia Identifies BfmAK System Control of Biofilm Development.Applied and environmental microbiology · 2016Article
- Characterization of the general stress response in Bartonella henselae.Microbial pathogenesis · 2016Article
- Multiple σEcfG and NepR Proteins Are Involved in the General Stress Response in Methylobacterium extorquens.PloS one · 2016Article
- Two-tiered histidine kinase pathway involved in heat shock and salt sensing in the general stress response of Sphingomonas melonis Fr1.Journal of bacteriology · 2015Article
- General Stress Signaling in the Alphaproteobacteria.Annual review of genetics · 2015Review
- LOV Histidine Kinase Modulates the General Stress Response System and Affects the virB Operon Expression in Brucella abortus.PloS one · 2015Article
- Complex two-component signaling regulates the general stress response in Alphaproteobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The Brucella abortus virulence regulator, LovhK, is a sensor kinase in the general stress response signalling pathway.Molecular microbiology · 2014Article
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2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The EcfG-type sigma factor RpoE2 is the regulator of the general stress response in Sinorhizobium meliloti. RpoE2 activity is negatively regulated by two NepR-type anti-sigma factors (RsiA1/A2), themselves under the control of two anti-anti-sigma factors (RsiB1/B2) belonging to the PhyR family of response regulators. The current model of RpoE2 activation suggests that in response to stress, RsiB1/B2 are activated by phosphorylation of an aspartate residue in their receiver domain. Once activated, RsiB1/B2 become able to interact with the anti-sigma factors and release RpoE2, which can then associate with the RNA polymerase to transcribe its target genes. The purpose of this work was to identify and characterize proteins involved in controlling the phosphorylation status of RsiB1/B2. Using in vivo approaches, we show that the putative histidine kinase encoded by the rsiC gene (SMc01507), located downstream from rpoE2, is able to both positively and negatively regulate the general stress response. In addition, our data suggest that the negative action of RsiC results from inhibition of RsiB1/B2 phosphorylation. From these observations, we propose that RsiC is a bifunctional histidine kinase/phosphatase responsible for RsiB1/B2 phosphorylation or dephosphorylation in the presence or absence of stress, respectively. Two proteins were previously proposed to control PhyR phosphorylation in Caulobacter crescentus and Sphingomonas sp. strain FR1. However, these proteins contain a Pfam:HisKA_2 domain of dimerization and histidine phosphotransfer, whereas S. meliloti RsiC harbors a Pfam:HWE_HK domain instead. Therefore, this is the first report of an HWE_HK-containing protein controlling the general stress response in Alphaproteobacteria.
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