ArticleCurrent biology : CB2014
HSP70 transgene directed motion to nuclear speckles facilitates heat shock activation.
Article in Current biology : CB, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
101 citing papers in PubMed, 172 citations in OpenAlex.
- Temporal gating dictates stress-induced transcript export from the nucleus.Genes & development · 2026Article
- Nuclear speckles: a fundamental layer of gene regulation.Trends in cell biology · 2026Review
- The periphery of nuclear speckles defines a spatially and temporally regulated compartment of long-lived intron-retained RNAs that resolves during mitosis.Nature cell biology · 2026Article
- Nuclear speckle-associated perispeckle networks partition active chromatin into gene expression niches.Cell reports · 2026Article
- Genome folding and nuclear speckles converge to orchestrate fibroblast activation.Science advances · 2026Article
- Temporal gating dictates stress-induced transcript export from the nucleus.bioRxiv : the preprint server for biology · 2026Article
- The dynamics of nucleolus-centromeres interaction in living cells.bioRxiv : the preprint server for biology · 2026Article
- Article
- Decoding the functions of nuclear speckles in neurodegeneration.Trends in neurosciences · 2026Review
- Nuclear ribonucleoprotein condensates as platforms for gene expression regulation.Genes & genomics · 2025Review
- Endogenous Real Time Imaging Reveals Dynamic Chromosomal Mobility During Ligand-Mediated Transcriptional Burst Events.bioRxiv : the preprint server for biology · 2025Article
- Major nuclear locales define nuclear genome organization and function beyond A and B compartments.eLife · 2025Article
- RBBP6 anchors pre-mRNA 3' end processing to nuclear speckles for efficient gene expression.Molecular cell · 2025Article
- Nuclear speckles regulate functional programs in cancer.Nature cell biology · 2025Article
- Mini review: Studying epigenomic alterations can shed light on coping and adaptive abilities during heat stress in monogastric livestock.Frontiers in genetics · 2025Review
- Inter-chromosomal transcription hubs shape the 3D genome architecture of African trypanosomes.Nature communications · 2024Article
- Divergent Contribution of Cytoplasmic Actins to Nuclear Structure of Lung Cancer Cells.International journal of molecular sciences · 2024Article
- Nuclear speckle biology: At the cross-roads of discovery and functional analysis.Current opinion in cell biology · 2024Review
- Nuclear actin filaments - a historical perspective.Nucleus (Austin, Tex.) · 2024Review
- Dynamics of RNA localization to nuclear speckles are connected to splicing efficiency.Science advances · 2024Article
41 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on by
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Association and disassociation of gene loci with respect to specific nuclear compartments accompany changes in gene expression, yet little is known concerning the mechanisms by which this occurs or its functional consequences. Previously, we showed that tethering acidic activators to a peripheral chromosome site led to movement of the chromosome site away from the nuclear periphery, but the physiological relevance of this movement was unclear [1]. Nuclear speckles, or interchromatin granule clusters, are enriched in factors involved in RNA processing [2], and the association of a subset of active genes at their periphery suggests speckles may play a role in gene expression [3, 4]. Here, we show an actin-dependent association of HSP70 transgenes with nuclear speckles after heat shock. We visualized HSP70 transgenes moving curvilinearly toward nuclear speckles over ∼0.5-6 μm distances at velocities of 1-2 μm min(-1). Chromatin stretching in the direction of movement demonstrates a force-generating mechanism. Transcription in nearly all cases increased noticeably only after initial contact with a nuclear speckle. Moreover, blocking new HSP70 transgene/speckle association by actin depolymerization prevented significant heat shock-induced transcriptional activation in transgenes not associated with speckles, although robust transcriptional activation was observed for HSP70 transgenes associated with nuclear speckles. Our results demonstrate the existence of a still-to-be-revealed machinery for moving chromatin in a direct path over long distances toward nuclear speckles in response to transcriptional activation; moreover, this speckle association enhances the heat shock activation of these HSP70 transgenes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.