ArticleMolecular biotechnology2014
Recombinant cellulase accumulation in the leaves of mature, vegetatively propagated transgenic sugarcane.
Article in Molecular biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- Pro-Vitamin A Biofortified Cavendish Banana: Trait Stability in the Field.Plant biotechnology journal · 2026Article
- Expression of a Hyperthermophilic Cellobiohydrolase in TransgenicPlants (Basel, Switzerland) · 2020Article
- Unprecedented enhancement of recombinant protein production in sugarcane culms using a combinatorial promoter stacking system.Scientific reports · 2020Article
- High-Level Production of Recombinant Snowdrop Lectin in Sugarcane and Energy Cane.Frontiers in bioengineering and biotechnology · 2020Article
- In planta expression of hyperthermophilic enzymes as a strategy for accelerated lignocellulosic digestion.Scientific reports · 2017Article
- Genetic engineering of grass cell wall polysaccharides for biorefining.Plant biotechnology journal · 2017Review
- Vacuolar deposition of recombinant proteins in plant vegetative organs as a strategy to increase yields.Bioengineered · 2017Review
- Long-term T-DNA insert stability and transgene expression consistency in field propagated sugarcane.Plant molecular biology · 2017Article
- In planta production and characterization of a hyperthermostable GH10 xylanase in transgenic sugarcane.Plant molecular biology · 2017Article
- Precision breeding for RNAi suppression of a major 4-coumarate:coenzyme A ligase gene improves cell wall saccharification from field grown sugarcane.Plant molecular biology · 2016Article
- Strategies for the production of cell wall-deconstructing enzymes in lignocellulosic biomass and their utilization for biofuel production.Plant biotechnology journal · 2016Review
- Vacuolar targeting of r-proteins in sugarcane leads to higher levels of purifiable commercially equivalent recombinant proteins in cane juice.Plant biotechnology journal · 2016Article
- Challenges and advances in the heterologous expression of cellulolytic enzymes: a review.Biotechnology for biofuels · 2014Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cost of enzymes that hydrolyse lignocellulosic substrates to fermentable sugars needs to be reduced to make cellulosic ethanol a cost-competitive liquid transport fuel. Sugarcane is a perennial crop and the successful integration of cellulase transgenes into the sugarcane production system requires that transgene expression is stable in the ratoon. Herein, we compared the accumulation of recombinant fungal cellobiohydrolase I (CBH I), fungal cellobiohydrolase II (CBH II), and bacterial endoglucanase (EG) in the leaves of mature, initial transgenic sugarcane plants and their mature ratoon. Mature ratoon events containing equivalent or elevated levels of active CBH I, CBH II, and EG in the leaves were identified. Further, we have demonstrated that recombinant fungal CBH I and CBH II can resist proteolysis during sugarcane leaf senescence, while bacterial EG cannot. These results demonstrate the stability of cellulase enzyme transgene expression in transgenic sugarcane and the utility of sugarcane as a biofactory crop for production of cellulases.
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