ArticleAmerican journal of human genetics1989
Chromosome heteromorphism quantified by high-resolution bivariate flow karyotyping.
Article in American journal of human genetics, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 69 citations in OpenAlex.
- Marsupial chromosome DNA content and genome size assessed from flow karyotypes: invariable low autosomal GC content.Royal Society open science · 2018Article
- De novo formed satellite DNA-based mammalian artificial chromosomes and their possible applications.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- The bat genome: GC-biased small chromosomes associated with reduction in genome size.Chromosoma · 2013Article
- Reassessment of genome size in turtle and crocodile based on chromosome measurement by flow karyotyping: close similarity to chicken.Biology letters · 2012Article
- Article
- Use of fluorescent sequence-specific polyamides to discriminate human chromosomes by microscopy and flow cytometry.Nucleic acids research · 2002Article
- All genomes great and small.Nature · 2002Article
- Article
- Standardization of bivariate flow karyotypes of human chromosomes for clinical applications.Journal of clinical laboratory analysis · 1997Article
- Chromosome-specific paints from a high-resolution flow karyotype of the dog.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1996Article
- Evidence for the organization of chromatin in megabase pair-sized loops arranged along a random walk path in the human G0/G1 interphase nucleus.The Journal of cell biology · 1995Article
- The 18q- syndrome: analysis of chromosomes by bivariate flow karyotyping and the PCR reveals a successive set of deletion breakpoints within 18q21.2-q22.2.American journal of human genetics · 1995Article
- Xp21 contiguous gene syndromes: deletion quantitation with bivariate flow karyotyping allows mapping of patient breakpoints.American journal of human genetics · 1992Article
- Molecular characterization of a patient with del(1)(q23-q25).Human genetics · 1991Article
- The male-derived genome after sperm-egg fusion: spatial distribution of chromosomal DNA and paternal-maternal genomic association.Chromosoma · 1991Article
- Inheritance of chromosome heteromorphisms analyzed by high-resolution bivariate flow karyotyping.American journal of human genetics · 1989Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Maternal and paternal homologues of many chromosome types can be differentiated on the basis of their peak position in Hoechst 33258 versus chromomycin A3 bivariate flow karyotypes. We demonstrate here the magnitude of DNA content differences among normal chromosomes of the same type. Significant peak-position differences between homologues were observed for an average of four chromosome types in each of the karyotypes of 98 different individuals. The frequency of individuals with differences in homologue peak positions varied among chromosome types: e.g., chromosome 15, 61%; chromosome 3, 4%. Flow karyotypes of 33 unrelated individuals were compared to determine the range of peak position among normal chromosomes. Chromosomes Y, 21, 22, 15, 16, 13, 14, and 19 were most heteromorphic, and chromosomes 2-8 and X were least heteromorphic. The largest chromosome 21 was 45% larger than the smallest 21 chromosome observed. The base composition of the variable regions differed among chromosome types. DNA contents of chromosome variants determined from flow karyotypes were closely correlated to measurements of DNA content made of gallocyanin chrome alum-stained metaphase chromosomes on slides. Fluorescence in situ hybridization with chromosome-specific repetitive sequences indicated that variability in their copy number is partly responsible for peak-position variability in some chromosomes. Heteromorphic chromosomes are identified for which parental flow karyotype information will be essential if de novo rearrangements resulting in small DNA content changes are to be detected with flow karyotyping.
Indexed as
Identifiers
2479266PMC1683437W1823395What OpenQuestion holds
Registered trials
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