ArticlePloS one2014
Maternal inheritance of twist and analysis of MAPK activation in embryos of the polychaete annelid Platynereis dumerilii.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 60 citations in OpenAlex.
- Evidence of autonomous neural specification for both brain and ventral nerve cord tissue in Annelida.bioRxiv : the preprint server for biology · 2026Article
- Functional evidence that FGFR regulates MAPK signaling in organizer specification in the gastropod molluskMarine life science & technology · 2023Article
- Transcriptomic landscape of posterior regeneration in the annelid Platynereis dumerilii.BMC genomics · 2023Article
- Remodeling of theBiology · 2023Article
- Early mesodermal development in the patellogastropodEvolutionary applications · 2023Article
- Patient-associated mutations in Drosophila Alk perturb neuronal differentiation and promote survival.Disease models & mechanisms · 2022Article
- ERK1/2 is an ancestral organising signal in spiral cleavage.Nature communications · 2022Article
- The Nereid on the rise: Platynereis as a model system.EvoDevo · 2021Review
- HES and Mox genes are expressed during early mesoderm formation in a mollusk with putative ancestral features.Scientific reports · 2021Article
- Behavioral variation according to feeding organ diversification in glossiphoniid leeches (Phylum: Annelida).Scientific reports · 2021Article
- FGF signaling acts on different levels of mesoderm development within Spiralia.Development (Cambridge, England) · 2021Article
- The development of early pioneer neurons in the annelid Malacoceros fuliginosus.BMC evolutionary biology · 2020Article
- Activin/Nodal signaling mediates dorsal-ventral axis formation before third quartet formation in embryos of the annelidEvoDevo · 2020Article
- From spiral cleavage to bilateral symmetry: the developmental cell lineage of the annelid brain.BMC biology · 2019Article
- Article
- A transcriptional blueprint for a spiral-cleaving embryo.BMC genomics · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we aimed to identify molecular mechanisms involved in the specification of the 4d (mesentoblast) lineage in Platynereis dumerilii. We employ RT-PCR and in situ hybridization against the Platynereis dumerilii twist homolog (Pdu-twist) to reveal mesodermal specification within this lineage. We show that Pdu-twist mRNA is already maternally distributed. After fertilization, ooplasmatic segregation leads to relocation of Pdu-twist transcripts into the somatoblast (2d) lineage and 4d, indicating that the maternal component of Pdu-twist might be an important prerequisite for further mesoderm specification but does not represent a defining characteristic of the mesentoblast. However, after the primordial germ cells have separated from the 4d lineage, zygotic transcription of Pdu-twist is exclusively observed in the myogenic progenitors, suggesting that mesodermal specification occurs after the 4d stage. Previous studies on spiral cleaving embryos revealed a spatio-temporal correlation between the 4d lineage and the activity of an embryonic organizer that is capable to induce the developmental fates of certain micromeres. This has raised the question if specification of the 4d lineage could be connected to the organizer activity. Therefore, we aimed to reveal the existence of such a proposed conserved organizer in Platynereis employing antibody staining against dpERK. In contrast to former observations in other spiralian embryos, activation of MAPK signaling during 2d and 4d formation cannot be detected which questions the existence of a conserved connection between organizer function and specification of the 4d lineage. However, our experiments unveil robust MAPK activation in the prospective nephroblasts as well as in the macromeres and some micromeres at the blastopore in gastrulating embryos. Inhibition of MAPK activation leads to larvae with a shortened body axis, defects in trunk muscle spreading and improper nervous system condensation, indicating a critical function for MAPK signaling for the reorganization of embryonic tissues during the gastrulation process.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.