ArticleNucleic acids research2014
Engineered zinc-finger transcription factors activate OCT4 (POU5F1), SOX2, KLF4, c-MYC (MYC) and miR302/367.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Effects of Organic Zinc Supplementation on Gut Microbiota and Health-Related Biomarkers in Healthy Adult Dogs.Biological trace element research · 2026Article
- Targeted epigenetic repression of a lymphoma oncogene by sequence-specific histone modifiers induces apoptosis in DLBCL.Leukemia & lymphoma · 2017Article
- Binding Site Configurations Probe the Structure and Dynamics of the Zinc Finger of NEMO (NF-κB Essential Modulator).Biochemistry · 2017Article
- Advanced Gene Manipulation Methods for Stem Cell Theranostics.Theranostics · 2017Review
- Editing the epigenome: technologies for programmable transcription and epigenetic modulation.Nature methods · 2016Review
- Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators.Scientific reports · 2016Article
- Enabling functional genomics with genome engineering.Genome research · 2015Review
- Genome-editing tools for stem cell biology.Cell death & disease · 2015Review
- Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers.Nature biotechnology · 2015Article
- Interrogation of a context-specific transcription factor network identifies novel regulators of pluripotency.Stem cells (Dayton, Ohio) · 2015Article
- Analysis of an artificial zinc finger epigenetic modulator: widespread binding but limited regulation.Nucleic acids research · 2014Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Artificial transcription factors are powerful tools for regulating gene expression. Here we report results with engineered zinc-finger transcription factors (ZF-TFs) targeting four protein-coding genes, OCT4, SOX2, KLF4 and c-MYC, and one noncoding ribonucleic acid (RNA) gene, the microRNA (miRNA) miR302/367 cluster. We designed over 300 ZF-TFs whose targets lie within 1 kb of the transcriptional start sites (TSSs), screened them for increased messenger RNA or miRNA levels in transfected cells, and identified potent ZF-TF activators for each gene. Furthermore, we demonstrate that selected ZF-TFs function with alternative activation domains and in multiple cell lines. For OCT4, we expanded the target range to -2.5 kb and +500 bp relative to the TSS and identified additional active ZF-TFs, including three highly active ZF-TFs targeting distal enhancer, proximal enhancer and downstream from the proximal promoter. Chromatin immunoprecipitation (FLAG-ChIP) results indicate that several inactive ZF-TFs targeting within the same regulatory region bind as well as the most active ZF-TFs, suggesting that efficient binding within one of these regulatory regions may be necessary but not sufficient for activation. These results further our understanding of ZF-TF design principles and corroborate the use of ZF-TFs targeting enhancers and downstream from the TSS for transcriptional activation.
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