ArticleNucleic acids research2014
Repeat 1 of TAL effectors affects target specificity for the base at position zero.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 41 citations in OpenAlex.
- Two TAL Effectors of Xanthomonas citri pv. malvacearum Induce Water Soaking by Activating GhSWEET14 Genes in Cotton.Molecular plant pathology · 2025Article
- Unconstrained Precision Mitochondrial Genome Editing with αDdCBEs.Human gene therapy · 2024Article
- Designer TALEs enable discovery of cell death-inducer genes.Plant physiology · 2024Article
- A single promoter-TALE system for tissue-specific and tuneable expression of multiple genes in rice.Plant biotechnology journal · 2022Article
- Epigenetic features improve TALE target prediction.BMC genomics · 2021Article
- Divalent cations promote TALE DNA-binding specificity.Nucleic acids research · 2020Article
- PrediTALE: A novel model learned from quantitative data allows for new perspectives on TALE targeting.PLoS computational biology · 2019Article
- Split-TALE: A TALE-Based Two-Component System for Synthetic Biology Applications in Planta.Plant physiology · 2019Article
- Functional instability allows access to DNA in longer transcription Activator-Like effector (TALE) arrays.eLife · 2019Article
- Selective recognition ofPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Article
- Engineering altered protein-DNA recognition specificity.Nucleic acids research · 2018Review
- Engineering DNA Backbone Interactions Results in TALE Scaffolds with Enhanced 5-Methylcytosine Selectivity.Scientific reports · 2017Article
- The effect of increasing numbers of repeats on TAL effector DNA binding specificity.Nucleic acids research · 2017Article
- TAL effector driven induction of a SWEET gene confers susceptibility to bacterial blight of cotton.Nature communications · 2017Article
- Modification of the PthA4 effector binding elements in Type I CsLOB1 promoter using Cas9/sgRNA to produce transgenic Duncan grapefruit alleviating XccΔpthA4:dCsLOB1.3 infection.Plant biotechnology journal · 2016Article
- AnnoTALE: bioinformatics tools for identification, annotation, and nomenclature of TALEs from Xanthomonas genomic sequences.Scientific reports · 2016Article
- Exploiting the CRISPR/Cas9 PAM Constraint for Single-Nucleotide Resolution Interventions.PloS one · 2016Article
- Suppression ofFrontiers in plant science · 2016Article
- Context influences on TALE-DNA binding revealed by quantitative profiling.Nature communications · 2015Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
AvrBs3, the founding member of the Xanthomonas transcription-activator-like effectors (TALEs), is translocated into the plant cell where it localizes to the nucleus and acts as transcription factor. The DNA-binding domain of AvrBs3 consists of 17.5 nearly-identical 34 amino acid-repeats. Each repeat specifies binding to one base in the target DNA via amino acid residues 12 and 13 termed repeat variable diresidue (RVD). Natural target sequences of TALEs are generally preceded by a thymine (T0), which is coordinated by a tryptophan residue (W232) in a degenerated repeat upstream of the canonical repeats. To investigate the necessity of T0 and the conserved tryptophan for AvrBs3-mediated gene activation we tested TALE mutant derivatives on target sequences preceded by all possible four bases. In addition, we performed domain swaps with TalC from a rice pathogenic Xanthomonas because TalC lacks the tryptophan residue, and the TalC target sequence is preceded by cytosine. We show that T0 works best and that T0 specificity depends on the repeat number and overall RVD-composition. T0 and W232 appear to be particularly important if the RVD of the first repeat is HD ('rep1 effect'). Our findings provide novel insights into the mechanism of T0 recognition by TALE proteins and are important for TALE-based biotechnological applications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.