ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2014
Comparison of three PCR-based assays for the non-invasive diagnosis of malaria: detection of Plasmodium parasites in blood and saliva.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.
- Accuracy of malaria diagnostic tests performed on non-invasively collected samples: a systematic review and meta-analysis.BMJ global health · 2021Pooled it
- Noninvasive malaria detection beyond blood sampling.Sensors & diagnostics · 2026Review
- Revolution in malaria detection: unveiling current breakthroughs and tomorrow's possibilities in biomarker innovation.Annals of medicine and surgery (2012) · 2024Review
- Review
- Biology and epidemiology ofParasite epidemiology and control · 2023Review
- Urine and Saliva: Relevant Specimens for Malaria Diagnosis?Diagnostics (Basel, Switzerland) · 2022Review
- Development of Two-Tube Loop-Mediated Isothermal Amplification Assay for Differential Diagnosis ofDiagnostics (Basel, Switzerland) · 2021Article
- Development and Clinical Validation of Iso-IMRS: A Novel Diagnostic Assay forAnalytical chemistry · 2021Article
- Evaluation of Malaria Diagnostic Methods as a Key for Successful Control and Elimination Programs.Tropical medicine and infectious disease · 2020Review
- Detection of the Malaria causing Plasmodium Parasite in Saliva from Infected Patients using Topoisomerase I Activity as a Biomarker.Scientific reports · 2018Article
- malERA: An updated research agenda for diagnostics, drugs, vaccines, and vector control in malaria elimination and eradication.PLoS medicine · 2017Review
- Can Mixed Parasite Infections Thwart Targeted Malaria Elimination Program in India?BioMed research international · 2017Article
- Establishing a China malaria diagnosis reference laboratory network for malaria elimination.Malaria journal · 2015Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The conventional molecular diagnosis of malaria uses 18S rRNA-based PCR assay employing blood samples. This assay presents limitation in terms of long turnaround time and increased chances of false-positive results. Here, we evaluated one-step singleplex or multiplex PCR assay based on high copy species-specific consensus repeat sequences (CRS) along with standard 18S rRNA nested PCR (18S n-PCR) assay to detect P. falciparum and P. vivax infection using blood and saliva samples from Indian febrile patients. Out of 327 patients, 187 were found to be positive for malaria parasites by microscopic examination of peripheral blood smears. Among these 130 were P. vivax and 57 were P. falciparum cases. The 18S n-PCR assay and CRS PCR assay identified 186 out of 187 cases (99.4 %). Multiplex CRS PCR assay detected Plasmodium in 176 out of 187 cases (94.1 %). Both singleplex and multiplex CRS PCR assay identified 6 mixed infection cases, while 18S n-PCR assay detected 10 mixed infection cases of P. vivax and P. falciparum, which were not recognized by microscopy. Non-invasive Plasmodium detection rate with DNA derived from saliva samples was highest for 18S n-PCR (87.36 %), followed by singleplex CRS (81 %) and multiplex CRS PCR assay (70.5 %). Specificity for P. vivax and P. falciparum detection for all assays was 98.48 % and 100 % respectively. Detection rate for P. vivax in saliva correlated with parasite density for CRS target-based assays. The species-specific CRS PCR, either as a singleplex or multiplex assay, can have an impact on diagnosis and epidemiological studies in malaria.
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