ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2014
Acetylcholine controls GABA-, glutamate-, and glycine-dependent giant depolarizing potentials that govern spontaneous motoneuron activity at the onset of synaptogenesis in the mouse embryonic spinal cord.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- KCC2 phosphorylation dynamics relate to oligomerization during development and after spinal cord injury.Frontiers in molecular neuroscience · 2026Article
- Metallothionein III Mediates CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Isoform requirement of clustered protocadherin for preventing neuronal apoptosis and neonatal lethality.iScience · 2023Article
- Chloride Homeostasis in Developing Motoneurons.Advances in neurobiology · 2022Article
- Spontaneous, synchronous zinc spikes oscillate with neural excitability and calcium spikes in primary hippocampal neuron culture.Journal of neurochemistry · 2021Article
- Article
- Dynamic regulation of the cholinergic system in the spinal central nervous system.Scientific reports · 2020Article
- Synaptic Plasticity on Motoneurons After Axotomy: A Necessary Change in Paradigm.Frontiers in molecular neuroscience · 2020Review
- Cholinergic Modulation of Glial Function During Aging and Chronic Neuroinflammation.Frontiers in cellular neuroscience · 2020Review
- Persistent Sodium Current Drives Excitability of Immature Renshaw Cells in Early Embryonic Spinal Networks.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Comparative transcriptomic profiling of peripheral efferent and afferent nerve fibres at different developmental stages in mice.Scientific reports · 2018Article
- Giant Depolarizing Potentials Trigger Transient Changes in the Intracellular ClFrontiers in cellular neuroscience · 2018Article
- Microcircuit formation following transplantation of mouse embryonic stem cell-derived neurons in peripheral nerve.Journal of neurophysiology · 2017Article
- Tonic nicotinic transmission enhances spinal GABAergic presynaptic release and the frequency of spontaneous network activity.Developmental neurobiology · 2016Article
- Depolarizing GABA/glycine synaptic events switch from excitation to inhibition during frequency increases.Scientific reports · 2016Article
- Normal Molecular Specification and Neurodegenerative Disease-Like Death of Spinal Neurons Lacking the SNARE-Associated Synaptic Protein Munc18-1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Regulation of Spontaneous Propagating Waves in the Embryonic Mouse Brainstem.Frontiers in neural circuits · 2016Article
- Development of Spontaneous Activity in the Avian Hindbrain.Frontiers in neural circuits · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A remarkable feature of early neuronal networks is their endogenous ability to generate spontaneous rhythmic electrical activity independently of any external stimuli. In the mouse embryonic SC, this activity starts at an embryonic age of ∼ 12 d and is characterized by bursts of action potentials recurring every 2-3 min. Although these bursts have been extensively studied using extracellular recordings and are known to play an important role in motoneuron (MN) maturation, the mechanisms driving MN activity at the onset of synaptogenesis are still poorly understood. Because only cholinergic antagonists are known to abolish early spontaneous activity, it has long been assumed that spinal cord (SC) activity relies on a core network of MNs synchronized via direct cholinergic collaterals. Using a combination of whole-cell patch-clamp recordings and extracellular recordings in E12.5 isolated mouse SC preparations, we found that spontaneous MN activity is driven by recurrent giant depolarizing potentials. Our analysis reveals that these giant depolarizing potentials are mediated by the activation of GABA, glutamate, and glycine receptors. We did not detect direct nAChR activation evoked by ACh application on MNs, indicating that cholinergic inputs between MNs are not functional at this age. However, we obtained evidence that the cholinergic dependency of early SC activity reflects a presynaptic facilitation of GABA and glutamate synaptic release via nicotinic AChRs. Our study demonstrates that, even in its earliest form, the activity of spinal MNs relies on a refined poly-synaptic network and involves a tight presynaptic cholinergic regulation of both GABAergic and glutamatergic inputs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.