ArticleThe Journal of biological chemistry2014
Blue light-induced dimerization of monomeric aureochrome-1 enhances its affinity for the target sequence.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 47 citations in OpenAlex.
- Light-dependent flavin redox and adduct states control the conformation and DNA-binding activity of the transcription factor EL222.Nucleic acids research · 2025Article
- A rapid aureochrome opto-switch enables diatom acclimation to dynamic light.Nature communications · 2024Article
- More than a photoreceptor: aureochromes are intrinsic to the diatom light-regulated transcriptional network.Journal of experimental botany · 2024Article
- Article
- Dimerization processes for light-regulated transcription factor Photozipper visualized by high-speed atomic force microscopy.Scientific reports · 2022Article
- Light-Inducible Spatio-Temporal Control of TLR4 and NF-κB-Gluc Reporter in Human Pancreatic Cell Line.International journal of molecular sciences · 2021Article
- Transmission of light signals from the light-oxygen-voltage core via the hydrophobic region of the β-sheet surface in aureochrome-1.Scientific reports · 2021Article
- A Time-Resolved Diffusion Technique for Detection of the Conformational Changes and Molecular Assembly/Disassembly Processes of Biomolecules.Frontiers in genetics · 2021Review
- The Aureochrome Photoreceptor PtAUREO1a Is a Highly Effective Blue Light Switch in Diatoms.iScience · 2020Article
- Deciphering the Allosteric Process of theThe journal of physical chemistry. B · 2020Article
- Exploring the FMN binding site in the mitochondrial outer membrane protein mitoNEET.Free radical biology & medicine · 2020Article
- Review
- Allosteric control of human cystathionine β-synthase activity by a redox active disulfide bond.The Journal of biological chemistry · 2018Article
- Quantitative analyses of the equilibria among DNA complexes of a blue-light-regulated bZIP module, Photozipper.Biophysics and physicobiology · 2018Article
- Illuminating developmental biology through photochemistry.Nature chemical biology · 2017Article
- Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes.Nucleic acids research · 2016Article
- Functional and topological diversity of LOV domain photoreceptors.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Blue-light-regulated transcription factor, Aureochrome, in photosynthetic stramenopiles.Journal of plant research · 2016Review
- Article
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5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aureochrome-1 (AUREO1) is a blue light (BL) receptor that mediates the branching response in stramenopile alga, Vaucheria frigida. AUREO1 contains a basic leucine zipper (bZIP) domain in the central region and a light-oxygen-voltage sensing (LOV) domain at the C terminus, and has been suggested to function as a light-regulated transcription factor. We have previously reported that preparations of recombinant AUREO1 contained the complete coding sequence (full-length, FL) and N-terminal truncated protein (ZL) containing bZIP and LOV domains, and suggested that wild-type ZL (ZLwt2) was in a dimer form with intermolecular disulfide linkages at Cys(162) and Cys(182) (Hisatomi, O., Takeuchi, K., Zikihara, K., Ookubo, Y., Nakatani, Y., Takahashi, F., Tokutomi, S., and Kataoka, H. (2013) Plant Cell Physiol. 54, 93-106). In the present study, we report the photoreactions, oligomeric structures, and DNA binding of monomeric cysteine to serine-mutated ZL (ZLC2S), DTT-treated ZL (DTT-ZL), and FL (DTT-FL). Recombinant AUREO1 showed similar spectral properties and dark regeneration kinetics to those of dimeric ZLwt2. Dynamic light scattering and size exclusion chromatography revealed that ZLC2S and DTT-ZL were monomeric in the dark state. Dissociation of intermolecular disulfide bonds of ZLwt2 was in equilibrium with a midpoint oxidation-redox potential of approximately -245 ± 15 mV. BL induced the dimerization of monomeric ZL, which subsequently increased its affinity for the target sequence. Also, DTT-FL was monomeric in the dark state and underwent BL-induced dimerization, which led to formation of the FL2·DNA complex. Taken together, our results suggest that monomeric AUREO1 is present in vivo, with dimerization playing a key role in its role as a BL-regulated transcription factor.
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