ArticleThe Journal of biological chemistry2014
Conserved modular domains team up to latch-open active protein kinase Cα.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 25 citations in OpenAlex.
- Optogenetic control of Protein Kinase C-epsilon activity reveals its intrinsic signaling properties with spatiotemporal resolution.bioRxiv : the preprint server for biology · 2025Article
- mTORC2 controls the activity of PKC and Akt by phosphorylating a conserved TOR interaction motif.Science signaling · 2021Article
- ER/K-link-Leveraging a native protein linker to probe dynamic cellular interactions.Methods in enzymology · 2021Review
- Activated Protein Kinase C (PKC) Is Persistently Trafficked with Epidermal Growth Factor (EGF) Receptor.Biomolecules · 2020Article
- Remarkable Rigidity of the Single α-Helical Domain of Myosin-VI As Revealed by NMR Spectroscopy.Journal of the American Chemical Society · 2019Article
- Bitopic Inhibition of ATP and Substrate Binding in Ser/Thr Kinases through a Conserved Allosteric Mechanism.Biochemistry · 2018Article
- A recurrent point mutation in PRKCA is a hallmark of chordoid gliomas.Nature communications · 2018Article
- Distinct structural mechanisms determine substrate affinity and kinase activity of protein kinase Cα.The Journal of biological chemistry · 2017Article
- ER/K linked GPCR-G protein fusions systematically modulate second messenger response in cells.Scientific reports · 2017Article
- The tumor promoter-activated protein kinase Cs are a system for regulating filopodia.Cytoskeleton (Hoboken, N.J.) · 2017Article
- The Role of Regulatory Domains in Maintaining Autoinhibition in the Multidomain Kinase PKCα.The Journal of biological chemistry · 2017Article
- C2-domain mediated nano-cluster formation increases calcium signaling efficiency.Scientific reports · 2016Article
- Patterning protein complexes on DNA nanostructures using a GFP nanobody.Protein science : a publication of the Protein Society · 2016Article
- Dynamic Response of the C2 Domain of Protein Kinase Cα to CaBiophysical journal · 2016Article
- Substrate Affinity Differentially Influences Protein Kinase C Regulation and Inhibitor Potency.The Journal of biological chemistry · 2016Article
- Tetraspanin CD82 Regulates the Spatiotemporal Dynamics of PKCα in Acute Myeloid Leukemia.Scientific reports · 2016Article
- Article
- Dynamics and Membrane Interactions of Protein Kinase C.Biochemistry · 2015Review
- Tuning the signalling output of protein kinase C.Biochemical Society transactions · 2014Review
- Förster resonance energy transfer - an approach to visualize the spatiotemporal regulation of macromolecular complex formation and compartmentalized cell signaling.Biochimica et biophysica acta · 2014Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Signaling proteins comprised of modular domains have evolved along with multicellularity as a method to facilitate increasing intracellular bandwidth. The effects of intramolecular interactions between modular domains within the context of native proteins have been largely unexplored. Here we examine intra- and intermolecular interactions in the multidomain signaling protein, protein kinase Cα (PKCα). We identify three interactions between two activated PKC molecules that synergistically stabilize a nanomolar affinity homodimer. Disruption of the homodimer results in a loss of PKC-mediated ERK1/2 phosphorylation, whereas disruption of the auto-inhibited state promotes the homodimer and prolongs PKC membrane localization. These observations support a novel regulatory mechanism wherein homodimerization dictates the equilibrium between the auto-inhibited and active states of PKC by sequestering auto-inhibitory interactions. Our findings underscore the physiological importance of context-dependent modular domain interactions in cell signaling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.