ArticleCellular and molecular neurobiology2014
3D porous chitosan scaffolds suit survival and neural differentiation of dental pulp stem cells.
Article in Cellular and molecular neurobiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 55 citations in OpenAlex.
- Sustainable nanomaterials for precision dental medicine: green synthesis, therapeutic applications, and future directions.Journal of nanobiotechnology · 2026Review
- Molecular Mechanisms and Clinical Applications of Neural Regeneration Through Dental Pulp Stem Cells.Stem cells international · 2026Review
- Immunomodulatory natural polysaccharide-based nanoparticles for the treatment of neurodegenerative diseases.Ibrain · 2025Review
- A Comparison Between Three Types of Scaffolds for Pulp Regeneration: A Histological Study on Dogs.Clinical and experimental dental research · 2024Article
- A Promising Application of Injectable Hydrogels in Nerve Repair and Regeneration for Ischemic Stroke.International journal of nanomedicine · 2024Review
- The Effect of Biomaterials on Human Dental Pulp Stem Cell Neural Differentiation: A Scoping Review.Cell journal · 2023Article
- Scaffolds for Dentin-Pulp Complex Regeneration.Medicina (Kaunas, Lithuania) · 2023Review
- Application of Dental Pulp Stem Cells for Bone and Neural Tissue Regeneration in Oral and Maxillofacial Region.Stem cells international · 2023Review
- Macro, Micro, and Nano-Inspired Bioactive Polymeric Biomaterials in Therapeutic, and Regenerative Orofacial Applications.Drug design, development and therapy · 2023Review
- Neural Regeneration in Regenerative Endodontic Treatment: An Overview and Current Trends.International journal of molecular sciences · 2022Review
- Biomaterial scaffolds for clinical procedures in endodontic regeneration.Bioactive materials · 2022Article
- Advances on Hydrogels for Oral Science Research.Gels (Basel, Switzerland) · 2022Review
- MTA-Enriched Polymeric Scaffolds Enhanced the Expression of Angiogenic Markers in Human Dental Pulp Stem Cells.Stem cells international · 2022Article
- Neuronal Cell Differentiation of Human Dental Pulp Stem Cells on Synthetic Polymeric Surfaces Coated With ECM Proteins.Frontiers in cell and developmental biology · 2022Article
- Self-Assembling Hydrogel Structures for Neural Tissue Repair.ACS biomaterials science & engineering · 2021Review
- Peripheral-neuron-like properties of differentiated human dental pulp stem cells (hDPSCs).PloS one · 2021Article
- Dental stem cells: The role of biomaterials and scaffolds in developing novel therapeutic strategies.World journal of stem cells · 2020Review
- Injectable Biomaterials for Dental Tissue Regeneration.International journal of molecular sciences · 2020Review
- An update on human periapical cyst-mesenchymal stem cells and their potential applications in regenerative medicine.Molecular biology reports · 2020Review
- Potential of Chitosan and Its Derivatives for Biomedical Applications in the Central Nervous System.Frontiers in bioengineering and biotechnology · 2020Review
Corrections and comments
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Authors and funding
14 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A key aspect of cell replacement therapy in brain injury treatment is construction of a suitable biomaterial scaffold that can effectively carry and transport the therapeutic cells to the target area. In the present study, we created small 3D porous chitosan scaffolds through freeze-drying, and showed that these can support and enhance the differentiation of dental pulp stem cells (DPSCs) to nerve cells in vitro. The DPSCs were collected from the dental pulp of adult human third molars. At a swelling rate of ~84.33 ± 10.92 %, the scaffold displayed high porosity and interconnectivity of pores, as revealed by SEM. Cell counting kit-8 assay established the biocompatibility of the chitosan scaffold, supporting the growth and survival of DPSCs. The successful neural differentiation of DPSCs was assayed by RT-PCR, western blotting, and immunofluorescence. We found that the scaffold-attached DPSCs showed high expression of Nestin that decreased sharply following induction of differentiation. Exposure to the differentiation media also increased the expression of neural molecular markers Microtubule-associated protein 2, glial fibrillary acidic protein, and 2',3'-cyclic nucleotide phosphodiesterase. This study demonstrates that the granular 3D chitosan scaffolds are non-cytotoxic, biocompatible, and provide a conducive and favorable micro-environment for attachment, survival, and neural differentiation of DPSCs. These scaffolds have enormous potential to facilitate future advances in treatment of brain injury.
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